Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies

Oil palm (Elaeis guineensis Jacq.) is the highest yielding oil-bearing crop. However, being a perennial crop, genetic improvement of oil palm is extremely slow. Indeed, compared to other annual oil crops such as soybean and rapeseed, genetic manipulations remained less important. Therefore, to remai...

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Autores principales:	Abdullah,Ruslan, Zainal,Alizah, Yew Heng,Wee, Chui Li,Leaw, Chee Beng,Yeap, Mei Phing,Lee, Abdullah Sirajuddin,Salwa, Soo Ping,Winnie Yap, Lourdes Joseph,Juanita, Azma Jusoh,Siti
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2005
Materias:	genetic transformation studies immature embryos in vitro culture oil palm plant regeneration
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100006
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spelling	oai:scielo:S0717-345820050001000062005-08-17Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studiesAbdullah,RuslanZainal,AlizahYew Heng,WeeChui Li,LeawChee Beng,YeapMei Phing,LeeAbdullah Sirajuddin,SalwaSoo Ping,Winnie YapLourdes Joseph,JuanitaAzma Jusoh,Siti genetic transformation studies immature embryos in vitro culture oil palm plant regeneration Oil palm (Elaeis guineensis Jacq.) is the highest yielding oil-bearing crop. However, being a perennial crop, genetic improvement of oil palm is extremely slow. Indeed, compared to other annual oil crops such as soybean and rapeseed, genetic manipulations remained less important. Therefore, to remain competitive, oil palm growers and breeders need new and novel approaches. In this report, the potential of immature embryos (IE) as a useful tool for oil palm genetic transformation studies was evaluated. It was evident that IEs were amenable to both direct and Agrobacterium-mediated gene transfer. Due to the abundant supply of IE, optimization of biolistic and Agrobacterium-mediated gene transfer into IEs were easily carried out. Transient transformation frequencies were comparable to other plant systems reported, with as high as 97.4% recorded for biolistic and 64.4% for Agrobacterium-mediated gene transfer. Like most moncots, oil palm tissues were less sensitive to kanamycin, geneticin and chloramphenicol. Instead, both hygromycin and phosphinotrycin were toxic 20 mg/l, making both suitable candidates for selecting putative transformants. IEs were also more responsive to in vitro manipulations as compared to other explants such as leaf and root tissues. Rapid in vitro response to callusing and embryogenesis or rapid and highly efficient direct germination resulted in a shorter culture period. This would minimize the production of abnormal clonal palms, which has been associated to chromosomal aberration due to prolonged time in culture. In addition, IEs also allows rapid and direct introduction of elite genes into breeding programs and in biclonal seed production.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.8 n.1 20052005-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100006en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	genetic transformation studies immature embryos in vitro culture oil palm plant regeneration
spellingShingle	genetic transformation studies immature embryos in vitro culture oil palm plant regeneration Abdullah,Ruslan Zainal,Alizah Yew Heng,Wee Chui Li,Leaw Chee Beng,Yeap Mei Phing,Lee Abdullah Sirajuddin,Salwa Soo Ping,Winnie Yap Lourdes Joseph,Juanita Azma Jusoh,Siti Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies
description	Oil palm (Elaeis guineensis Jacq.) is the highest yielding oil-bearing crop. However, being a perennial crop, genetic improvement of oil palm is extremely slow. Indeed, compared to other annual oil crops such as soybean and rapeseed, genetic manipulations remained less important. Therefore, to remain competitive, oil palm growers and breeders need new and novel approaches. In this report, the potential of immature embryos (IE) as a useful tool for oil palm genetic transformation studies was evaluated. It was evident that IEs were amenable to both direct and Agrobacterium-mediated gene transfer. Due to the abundant supply of IE, optimization of biolistic and Agrobacterium-mediated gene transfer into IEs were easily carried out. Transient transformation frequencies were comparable to other plant systems reported, with as high as 97.4% recorded for biolistic and 64.4% for Agrobacterium-mediated gene transfer. Like most moncots, oil palm tissues were less sensitive to kanamycin, geneticin and chloramphenicol. Instead, both hygromycin and phosphinotrycin were toxic 20 mg/l, making both suitable candidates for selecting putative transformants. IEs were also more responsive to in vitro manipulations as compared to other explants such as leaf and root tissues. Rapid in vitro response to callusing and embryogenesis or rapid and highly efficient direct germination resulted in a shorter culture period. This would minimize the production of abnormal clonal palms, which has been associated to chromosomal aberration due to prolonged time in culture. In addition, IEs also allows rapid and direct introduction of elite genes into breeding programs and in biclonal seed production.
author	Abdullah,Ruslan Zainal,Alizah Yew Heng,Wee Chui Li,Leaw Chee Beng,Yeap Mei Phing,Lee Abdullah Sirajuddin,Salwa Soo Ping,Winnie Yap Lourdes Joseph,Juanita Azma Jusoh,Siti
author_facet	Abdullah,Ruslan Zainal,Alizah Yew Heng,Wee Chui Li,Leaw Chee Beng,Yeap Mei Phing,Lee Abdullah Sirajuddin,Salwa Soo Ping,Winnie Yap Lourdes Joseph,Juanita Azma Jusoh,Siti
author_sort	Abdullah,Ruslan
title	Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies
title_short	Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies
title_full	Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies
title_fullStr	Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies
title_full_unstemmed	Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies
title_sort	immature embryo: a useful tool for oil palm (elaeis guineensis jacq.) genetic transformation studies
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100006
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Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies

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