Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers

The strain Chryseobacterium sp. kr6 shown to be useful for biotechnological purposes such as hydrolysis of poultry feathers and de-hairing of bovine pelts. The effect of temperature, initial pH and media composition on protease production by this keratinolytic strain was studied. The enzyme was prod...

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Autores principales: Brandelli,Adriano, Riffel,Alessandro
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2005
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100007
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spelling oai:scielo:S0717-345820050001000072005-08-17Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathersBrandelli,AdrianoRiffel,Alessandro bacteria enzyme keratin protease The strain Chryseobacterium sp. kr6 shown to be useful for biotechnological purposes such as hydrolysis of poultry feathers and de-hairing of bovine pelts. The effect of temperature, initial pH and media composition on protease production by this keratinolytic strain was studied. The enzyme was produced between 25 and 37ºC, with maximum activity and yield at 30ºC. When protease production was tested in media with different initial pH, maximum activity was observed when cultivation was carried out at 30ºC and initial pH ranging from 6.0 to 8.0. Higher activity was observed when feathers or feather meal were used as growth substrates, followed by soybean meal. The addition of carbohydrates or surfactants to feather broth resulted in decrease in keratinolytic activity.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.8 n.1 20052005-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100007en
institution Scielo Chile
collection Scielo Chile
language English
topic bacteria
enzyme
keratin
protease
spellingShingle bacteria
enzyme
keratin
protease
Brandelli,Adriano
Riffel,Alessandro
Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers
description The strain Chryseobacterium sp. kr6 shown to be useful for biotechnological purposes such as hydrolysis of poultry feathers and de-hairing of bovine pelts. The effect of temperature, initial pH and media composition on protease production by this keratinolytic strain was studied. The enzyme was produced between 25 and 37ºC, with maximum activity and yield at 30ºC. When protease production was tested in media with different initial pH, maximum activity was observed when cultivation was carried out at 30ºC and initial pH ranging from 6.0 to 8.0. Higher activity was observed when feathers or feather meal were used as growth substrates, followed by soybean meal. The addition of carbohydrates or surfactants to feather broth resulted in decrease in keratinolytic activity.
author Brandelli,Adriano
Riffel,Alessandro
author_facet Brandelli,Adriano
Riffel,Alessandro
author_sort Brandelli,Adriano
title Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers
title_short Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers
title_full Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers
title_fullStr Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers
title_full_unstemmed Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers
title_sort production of an extracellular keratinase from chryseobacterium sp. growing on raw feathers
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2005
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100007
work_keys_str_mv AT brandelliadriano productionofanextracellularkeratinasefromchryseobacteriumspgrowingonrawfeathers
AT riffelalessandro productionofanextracellularkeratinasefromchryseobacteriumspgrowingonrawfeathers
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