Sucrose hydrolysis catalyzed by auto-immobilized invertase into intact cells of Cladosporium cladosporioides
The enzyme known as invertase (E.C. 3.2.1.26 - β-D-fructofuranosidase) catalyzes the sucrose hydrolysis producing an equimolar mixture of glucose and fructose named inverted sugar. The fungus Cladosporium cladosporioides has invertase as its constituent. Hence, its use as a natural immobili...
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Pontificia Universidad Católica de Valparaíso
2005
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oai:scielo:S0717-345820050001000092005-08-17Sucrose hydrolysis catalyzed by auto-immobilized invertase into intact cells of Cladosporium cladosporioidesSantana de Almeida,Ana CláudiaCosta de Araújo,LuciaresMendes Costa,AndressaMoraes de Abreu,César AugustoGomes de Andrade Lima,Maria AlicePerez Fernandez Palha,Maria de Los Angeles Cladosporium cladosporioides factorial planning invertase The enzyme known as invertase (E.C. 3.2.1.26 - β-D-fructofuranosidase) catalyzes the sucrose hydrolysis producing an equimolar mixture of glucose and fructose named inverted sugar. The fungus Cladosporium cladosporioides has invertase as its constituent. Hence, its use as a natural immobilized support for the invertase produces interesting results for the enzyme. The present work has the objective of determining the optimum operational conditions of auto-immobilized invertase, as well as its kinetic parameters (K M and Vmax). A complete 2³ factorial planning was done for the evaluation of such parameters. Temperature, pH and agitation level were the studied variables. The hydrolysis percentage was the monitored result. Batch tests in optimum conditions were done to determine the kinetic parameters. Temperature of 70ºC, pH 6 and agitation of 170 rpm were the established conditions for the hydrolysis process. The auto-immobilized invertase presented a K M of 447 mM and Vmax of 2,805 mmol/min.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.8 n.1 20052005-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100009en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Cladosporium cladosporioides factorial planning invertase |
| spellingShingle |
Cladosporium cladosporioides factorial planning invertase Santana de Almeida,Ana Cláudia Costa de Araújo,Luciares Mendes Costa,Andressa Moraes de Abreu,César Augusto Gomes de Andrade Lima,Maria Alice Perez Fernandez Palha,Maria de Los Angeles Sucrose hydrolysis catalyzed by auto-immobilized invertase into intact cells of Cladosporium cladosporioides |
| description |
The enzyme known as invertase (E.C. 3.2.1.26 - β-D-fructofuranosidase) catalyzes the sucrose hydrolysis producing an equimolar mixture of glucose and fructose named inverted sugar. The fungus Cladosporium cladosporioides has invertase as its constituent. Hence, its use as a natural immobilized support for the invertase produces interesting results for the enzyme. The present work has the objective of determining the optimum operational conditions of auto-immobilized invertase, as well as its kinetic parameters (K M and Vmax). A complete 2³ factorial planning was done for the evaluation of such parameters. Temperature, pH and agitation level were the studied variables. The hydrolysis percentage was the monitored result. Batch tests in optimum conditions were done to determine the kinetic parameters. Temperature of 70ºC, pH 6 and agitation of 170 rpm were the established conditions for the hydrolysis process. The auto-immobilized invertase presented a K M of 447 mM and Vmax of 2,805 mmol/min. |
| author |
Santana de Almeida,Ana Cláudia Costa de Araújo,Luciares Mendes Costa,Andressa Moraes de Abreu,César Augusto Gomes de Andrade Lima,Maria Alice Perez Fernandez Palha,Maria de Los Angeles |
| author_facet |
Santana de Almeida,Ana Cláudia Costa de Araújo,Luciares Mendes Costa,Andressa Moraes de Abreu,César Augusto Gomes de Andrade Lima,Maria Alice Perez Fernandez Palha,Maria de Los Angeles |
| author_sort |
Santana de Almeida,Ana Cláudia |
| title |
Sucrose hydrolysis catalyzed by auto-immobilized invertase into intact cells of Cladosporium cladosporioides |
| title_short |
Sucrose hydrolysis catalyzed by auto-immobilized invertase into intact cells of Cladosporium cladosporioides |
| title_full |
Sucrose hydrolysis catalyzed by auto-immobilized invertase into intact cells of Cladosporium cladosporioides |
| title_fullStr |
Sucrose hydrolysis catalyzed by auto-immobilized invertase into intact cells of Cladosporium cladosporioides |
| title_full_unstemmed |
Sucrose hydrolysis catalyzed by auto-immobilized invertase into intact cells of Cladosporium cladosporioides |
| title_sort |
sucrose hydrolysis catalyzed by auto-immobilized invertase into intact cells of cladosporium cladosporioides |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2005 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100009 |
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