The upstream sequence of the phycocyanin b subunit gene from Arthrospira platensis regulates expression of gfp gene in response to light intensity

In cyanobacteria, few details are known of the mechanisms through which the expression of the light-harvesting pigment c-phycocyanin is regulated. In the present study, a 419 bp upstream sequence of the phycocyanin b subunit (cpcB) gene from Arthrospira platensis FACHB341 was fused with green fluore...

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Autores principales:	Yongzhong,Lu, Zhang,Xuecheng
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2005
Materias:	cpcB gene deletion analysis light intensity Synechococcus transformation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100010
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spelling	oai:scielo:S0717-345820050001000102005-08-17The upstream sequence of the phycocyanin b subunit gene from Arthrospira platensis regulates expression of gfp gene in response to light intensityYongzhong,LuZhang,Xuecheng cpcB gene deletion analysis light intensity Synechococcus transformation In cyanobacteria, few details are known of the mechanisms through which the expression of the light-harvesting pigment c-phycocyanin is regulated. In the present study, a 419 bp upstream sequence of the phycocyanin b subunit (cpcB) gene from Arthrospira platensis FACHB341 was fused with green fluorescent protein (gfp) gene, and a heterologous reporting system was built up to investigate the influence of light intensity on the expression of gfp gene, and the regulation function of different region of the upstream sequence of cpcB gene. Results showed that the upstream sequence of cpcB gene could drive the expression of gfp gene in Synechococcus sp. strain PCC7942, and the expression was influenced by light intensity, the lower the light intensity, the higher the GFP level. Deletion analysis revealed that a light-responsive element was located in the region -276 to-218, a promoter sequence was in the region -85 to -1, and two positive cis elements were in the -419 to -276 and the -218 to -130 regions, respectivelyinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.8 n.1 20052005-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100010en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cpcB gene deletion analysis light intensity Synechococcus transformation
spellingShingle	cpcB gene deletion analysis light intensity Synechococcus transformation Yongzhong,Lu Zhang,Xuecheng The upstream sequence of the phycocyanin b subunit gene from Arthrospira platensis regulates expression of gfp gene in response to light intensity
description	In cyanobacteria, few details are known of the mechanisms through which the expression of the light-harvesting pigment c-phycocyanin is regulated. In the present study, a 419 bp upstream sequence of the phycocyanin b subunit (cpcB) gene from Arthrospira platensis FACHB341 was fused with green fluorescent protein (gfp) gene, and a heterologous reporting system was built up to investigate the influence of light intensity on the expression of gfp gene, and the regulation function of different region of the upstream sequence of cpcB gene. Results showed that the upstream sequence of cpcB gene could drive the expression of gfp gene in Synechococcus sp. strain PCC7942, and the expression was influenced by light intensity, the lower the light intensity, the higher the GFP level. Deletion analysis revealed that a light-responsive element was located in the region -276 to-218, a promoter sequence was in the region -85 to -1, and two positive cis elements were in the -419 to -276 and the -218 to -130 regions, respectively
author	Yongzhong,Lu Zhang,Xuecheng
author_facet	Yongzhong,Lu Zhang,Xuecheng
author_sort	Yongzhong,Lu
title	The upstream sequence of the phycocyanin b subunit gene from Arthrospira platensis regulates expression of gfp gene in response to light intensity
title_short	The upstream sequence of the phycocyanin b subunit gene from Arthrospira platensis regulates expression of gfp gene in response to light intensity
title_full	The upstream sequence of the phycocyanin b subunit gene from Arthrospira platensis regulates expression of gfp gene in response to light intensity
title_fullStr	The upstream sequence of the phycocyanin b subunit gene from Arthrospira platensis regulates expression of gfp gene in response to light intensity
title_full_unstemmed	The upstream sequence of the phycocyanin b subunit gene from Arthrospira platensis regulates expression of gfp gene in response to light intensity
title_sort	upstream sequence of the phycocyanin b subunit gene from arthrospira platensis regulates expression of gfp gene in response to light intensity
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100010
work_keys_str_mv	AT yongzhonglu theupstreamsequenceofthephycocyaninbsubunitgenefromarthrospiraplatensisregulatesexpressionofgfpgeneinresponsetolightintensity AT zhangxuecheng theupstreamsequenceofthephycocyaninbsubunitgenefromarthrospiraplatensisregulatesexpressionofgfpgeneinresponsetolightintensity AT yongzhonglu upstreamsequenceofthephycocyaninbsubunitgenefromarthrospiraplatensisregulatesexpressionofgfpgeneinresponsetolightintensity AT zhangxuecheng upstreamsequenceofthephycocyaninbsubunitgenefromarthrospiraplatensisregulatesexpressionofgfpgeneinresponsetolightintensity
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The upstream sequence of the phycocyanin b subunit gene from Arthrospira platensis regulates expression of gfp gene in response to light intensity

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