T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics

With the availability of complete genome sequences of several organisms, the focus has shifted from structural genomics to functional genomics, specifically in plants where the complete genomic sequences are becoming available i.e., Arabidopsis and rice. Agrobacterium mediated transformation which i...

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Autores principales:	Radhamony,Resmi Nath, Mohan Prasad,Anand, Srinivasan,Ramamurthy
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2005
Materias:	Arabidopsis functional genomics insertional mutagenesis promoter trapping T-DNA tagging
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100012
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spelling	oai:scielo:S0717-345820050001000122005-08-17T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomicsRadhamony,Resmi NathMohan Prasad,AnandSrinivasan,Ramamurthy Arabidopsis functional genomics insertional mutagenesis promoter trapping T-DNA tagging With the availability of complete genome sequences of several organisms, the focus has shifted from structural genomics to functional genomics, specifically in plants where the complete genomic sequences are becoming available i.e., Arabidopsis and rice. Agrobacterium mediated transformation which is exploited for transgenic technology is also being used as an effective mutagen and as a tool for functional genomics in higher plants. Besides the fact that the insertion of T-DNA element into a gene can lead to loss or gain of function, ingenious use of a variety of vectors have led to the identification of genes and regulatory elements in Arabidopsis. In this review, we highlight the progress made in the field of functional genomics of Arabidopsis using T-DNA tagging. Since this strategy has been very successfully employed in Arabidopsis and is now being extended to other plant species, we discuss the various vectors and experimental approaches employed to tag, identify and clone genes and promoter elements in Arabidopsis using T-DNA as a tool.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.8 n.1 20052005-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100012en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Arabidopsis functional genomics insertional mutagenesis promoter trapping T-DNA tagging
spellingShingle	Arabidopsis functional genomics insertional mutagenesis promoter trapping T-DNA tagging Radhamony,Resmi Nath Mohan Prasad,Anand Srinivasan,Ramamurthy T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics
description	With the availability of complete genome sequences of several organisms, the focus has shifted from structural genomics to functional genomics, specifically in plants where the complete genomic sequences are becoming available i.e., Arabidopsis and rice. Agrobacterium mediated transformation which is exploited for transgenic technology is also being used as an effective mutagen and as a tool for functional genomics in higher plants. Besides the fact that the insertion of T-DNA element into a gene can lead to loss or gain of function, ingenious use of a variety of vectors have led to the identification of genes and regulatory elements in Arabidopsis. In this review, we highlight the progress made in the field of functional genomics of Arabidopsis using T-DNA tagging. Since this strategy has been very successfully employed in Arabidopsis and is now being extended to other plant species, we discuss the various vectors and experimental approaches employed to tag, identify and clone genes and promoter elements in Arabidopsis using T-DNA as a tool.
author	Radhamony,Resmi Nath Mohan Prasad,Anand Srinivasan,Ramamurthy
author_facet	Radhamony,Resmi Nath Mohan Prasad,Anand Srinivasan,Ramamurthy
author_sort	Radhamony,Resmi Nath
title	T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics
title_short	T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics
title_full	T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics
title_fullStr	T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics
title_full_unstemmed	T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics
title_sort	t-dna insertional mutagenesis in arabidopsis: a tool for functional genomics
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100012
work_keys_str_mv	AT radhamonyresminath tdnainsertionalmutagenesisinarabidopsisatoolforfunctionalgenomics AT mohanprasadanand tdnainsertionalmutagenesisinarabidopsisatoolforfunctionalgenomics AT srinivasanramamurthy tdnainsertionalmutagenesisinarabidopsisatoolforfunctionalgenomics
_version_	1718441731400138752

T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics

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