A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database

Chickpea, a lesser-studied grain legume, is being investigated due to its taxonomic proximity with the model legume genome Medicago truncatula and its ability to endure and grow in relatively low soil water contents making it a model legume crop for the study of agronomic response to drought stress....

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Autores principales:	Jayashree,B, Buhariwalla,Hutokshi K, Shinde,Sanjeev, Crouch,Jonathan H
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2005
Materias:	cloning data mining drought avoidance drought tolerance EST database root traits stress
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200002
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spelling	oai:scielo:S0717-345820050002000022005-10-14A legume genomics resource: The Chickpea Root Expressed Sequence Tag DatabaseJayashree,BBuhariwalla,Hutokshi KShinde,SanjeevCrouch,Jonathan H cloning data mining drought avoidance drought tolerance EST database root traits stress Chickpea, a lesser-studied grain legume, is being investigated due to its taxonomic proximity with the model legume genome Medicago truncatula and its ability to endure and grow in relatively low soil water contents making it a model legume crop for the study of agronomic response to drought stress. Public databases currently contain very few sequences from chickpea associated with expression in root tissues. However, root traits are likely to be one of the most important components of drought tolerance in chickpea. Thus, we have generated a set of over 2800 chickpea expressed sequence tags (ESTs) from a library constructed after subtractive suppressive hybridization (SSH) of root tissue from two closely related chickpea genotypes possessing different sources of drought avoidance and tolerance (ICC4958 and Annigeri respectively). This database provides researchers in legume genomics with a major new resource for data mining associated with root traits and drought tolerance. This report describes the development and utilization of the database and provides the tools we have developed to facilitate the bioinformatics pipeline used for analysis of the ESTs in this database. We also discuss applications that have already been achieved using this resourceinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.8 n.2 20052005-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200002en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cloning data mining drought avoidance drought tolerance EST database root traits stress
spellingShingle	cloning data mining drought avoidance drought tolerance EST database root traits stress Jayashree,B Buhariwalla,Hutokshi K Shinde,Sanjeev Crouch,Jonathan H A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database
description	Chickpea, a lesser-studied grain legume, is being investigated due to its taxonomic proximity with the model legume genome Medicago truncatula and its ability to endure and grow in relatively low soil water contents making it a model legume crop for the study of agronomic response to drought stress. Public databases currently contain very few sequences from chickpea associated with expression in root tissues. However, root traits are likely to be one of the most important components of drought tolerance in chickpea. Thus, we have generated a set of over 2800 chickpea expressed sequence tags (ESTs) from a library constructed after subtractive suppressive hybridization (SSH) of root tissue from two closely related chickpea genotypes possessing different sources of drought avoidance and tolerance (ICC4958 and Annigeri respectively). This database provides researchers in legume genomics with a major new resource for data mining associated with root traits and drought tolerance. This report describes the development and utilization of the database and provides the tools we have developed to facilitate the bioinformatics pipeline used for analysis of the ESTs in this database. We also discuss applications that have already been achieved using this resource
author	Jayashree,B Buhariwalla,Hutokshi K Shinde,Sanjeev Crouch,Jonathan H
author_facet	Jayashree,B Buhariwalla,Hutokshi K Shinde,Sanjeev Crouch,Jonathan H
author_sort	Jayashree,B
title	A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database
title_short	A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database
title_full	A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database
title_fullStr	A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database
title_full_unstemmed	A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database
title_sort	legume genomics resource: the chickpea root expressed sequence tag database
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200002
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A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database

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