Expression of the antibody 14D9 in Nicotiana tabacum hairy roots

Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts...

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Autores principales:	Martínez,Carolina, Petruccelli,Silvana, Giulietti,Ana María, Alvarez,María Alejandra
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2005
Materias:	catalytic antibodies in vitro cultures molecular farming recombinant antibodies tobacco
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200006
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spelling	oai:scielo:S0717-345820050002000062005-10-14Expression of the antibody 14D9 in Nicotiana tabacum hairy rootsMartínez,CarolinaPetruccelli,SilvanaGiulietti,Ana MaríaAlvarez,María Alejandra catalytic antibodies in vitro cultures molecular farming recombinant antibodies tobacco Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 µg and 14 µg respectivelyinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.8 n.2 20052005-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200006en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	catalytic antibodies in vitro cultures molecular farming recombinant antibodies tobacco
spellingShingle	catalytic antibodies in vitro cultures molecular farming recombinant antibodies tobacco Martínez,Carolina Petruccelli,Silvana Giulietti,Ana María Alvarez,María Alejandra Expression of the antibody 14D9 in Nicotiana tabacum hairy roots
description	Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 µg and 14 µg respectively
author	Martínez,Carolina Petruccelli,Silvana Giulietti,Ana María Alvarez,María Alejandra
author_facet	Martínez,Carolina Petruccelli,Silvana Giulietti,Ana María Alvarez,María Alejandra
author_sort	Martínez,Carolina
title	Expression of the antibody 14D9 in Nicotiana tabacum hairy roots
title_short	Expression of the antibody 14D9 in Nicotiana tabacum hairy roots
title_full	Expression of the antibody 14D9 in Nicotiana tabacum hairy roots
title_fullStr	Expression of the antibody 14D9 in Nicotiana tabacum hairy roots
title_full_unstemmed	Expression of the antibody 14D9 in Nicotiana tabacum hairy roots
title_sort	expression of the antibody 14d9 in nicotiana tabacum hairy roots
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200006
work_keys_str_mv	AT martinezcarolina expressionoftheantibody14d9innicotianatabacumhairyroots AT petruccellisilvana expressionoftheantibody14d9innicotianatabacumhairyroots AT giuliettianamaria expressionoftheantibody14d9innicotianatabacumhairyroots AT alvarezmariaalejandra expressionoftheantibody14d9innicotianatabacumhairyroots
_version_	1718441733612634112

Expression of the antibody 14D9 in Nicotiana tabacum hairy roots

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