Peroxidase production from hairy root cultures of red beet (Beta vulgaris)

The genetically transformed roots of red beet have been shown, for the first time, to produce very high levels of peroxidase (POD; EC 1.11.1.7) accounting for 1.21 x 10(6) Units L-1. Of the ten clones established using different strains of Agrobacterium rhizogenes, one was that from the strain LMG-1...

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Autores principales:	Rudrappa,Thimmaraju, Neelwarne,Bhagyalakshmi, Kumar,Vinod, Lakshmanan,Venkatachalam, Venkataramareddy,Sreedhar Reddampalli, Aswathanarayana,Ravishankar Gokare
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2005
Materias:	Agrobacterium rhizogenes auxins elicitation T-DNA thermostability transformation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200008
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spelling	oai:scielo:S0717-345820050002000082005-10-14Peroxidase production from hairy root cultures of red beet (Beta vulgaris)Rudrappa,ThimmarajuNeelwarne,BhagyalakshmiKumar,VinodLakshmanan,VenkatachalamVenkataramareddy,Sreedhar ReddampalliAswathanarayana,Ravishankar Gokare Agrobacterium rhizogenes auxins elicitation T-DNA thermostability transformation The genetically transformed roots of red beet have been shown, for the first time, to produce very high levels of peroxidase (POD; EC 1.11.1.7) accounting for 1.21 x 10(6) Units L-1. Of the ten clones established using different strains of Agrobacterium rhizogenes, one was that from the strain LMG-150, three each from A 2/83, A 20/83 and A4. All the clones showed true integration of T-DNA when tested by PCR and Southern hybridization methods. Each clone differed significantly from the others in growth, hormone dependency and POD production where LMG-150 produced highest biomass (140 g FW L-1) as well as POD (ranging from 8000-9000 U g-1 FW and 1.18 x 10(6) U L-1 with a specific activity of 600 U mg-1 protein) on hormone-free medium, both in shake-flask as well as in bioreactor with a further enhancement to 1.21 x 10(6) U L-1 upon the addition of extra calcium chloride (5 mM). PAGE with active staining showed 4 distinct bands of Rm 0.06, 0.16, 0.25, 0.38 and 0.46 in the biomass and bands at Rm 0.06, 0.16, 0.25 and one extra band of Rm 0.575 in the spent medium where isozymes of Rm 0.38 and 0.46 were totally absent. The pH optima and other properties were grossly comparable with the standard horse-radish POD (HRP) with better thermal stability than HRP and therefore, the present source appears to offer a cheaper and additional alternative for the commercial production of PODinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.8 n.2 20052005-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200008en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Agrobacterium rhizogenes auxins elicitation T-DNA thermostability transformation
spellingShingle	Agrobacterium rhizogenes auxins elicitation T-DNA thermostability transformation Rudrappa,Thimmaraju Neelwarne,Bhagyalakshmi Kumar,Vinod Lakshmanan,Venkatachalam Venkataramareddy,Sreedhar Reddampalli Aswathanarayana,Ravishankar Gokare Peroxidase production from hairy root cultures of red beet (Beta vulgaris)
description	The genetically transformed roots of red beet have been shown, for the first time, to produce very high levels of peroxidase (POD; EC 1.11.1.7) accounting for 1.21 x 10(6) Units L-1. Of the ten clones established using different strains of Agrobacterium rhizogenes, one was that from the strain LMG-150, three each from A 2/83, A 20/83 and A4. All the clones showed true integration of T-DNA when tested by PCR and Southern hybridization methods. Each clone differed significantly from the others in growth, hormone dependency and POD production where LMG-150 produced highest biomass (140 g FW L-1) as well as POD (ranging from 8000-9000 U g-1 FW and 1.18 x 10(6) U L-1 with a specific activity of 600 U mg-1 protein) on hormone-free medium, both in shake-flask as well as in bioreactor with a further enhancement to 1.21 x 10(6) U L-1 upon the addition of extra calcium chloride (5 mM). PAGE with active staining showed 4 distinct bands of Rm 0.06, 0.16, 0.25, 0.38 and 0.46 in the biomass and bands at Rm 0.06, 0.16, 0.25 and one extra band of Rm 0.575 in the spent medium where isozymes of Rm 0.38 and 0.46 were totally absent. The pH optima and other properties were grossly comparable with the standard horse-radish POD (HRP) with better thermal stability than HRP and therefore, the present source appears to offer a cheaper and additional alternative for the commercial production of POD
author	Rudrappa,Thimmaraju Neelwarne,Bhagyalakshmi Kumar,Vinod Lakshmanan,Venkatachalam Venkataramareddy,Sreedhar Reddampalli Aswathanarayana,Ravishankar Gokare
author_facet	Rudrappa,Thimmaraju Neelwarne,Bhagyalakshmi Kumar,Vinod Lakshmanan,Venkatachalam Venkataramareddy,Sreedhar Reddampalli Aswathanarayana,Ravishankar Gokare
author_sort	Rudrappa,Thimmaraju
title	Peroxidase production from hairy root cultures of red beet (Beta vulgaris)
title_short	Peroxidase production from hairy root cultures of red beet (Beta vulgaris)
title_full	Peroxidase production from hairy root cultures of red beet (Beta vulgaris)
title_fullStr	Peroxidase production from hairy root cultures of red beet (Beta vulgaris)
title_full_unstemmed	Peroxidase production from hairy root cultures of red beet (Beta vulgaris)
title_sort	peroxidase production from hairy root cultures of red beet (beta vulgaris)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200008
work_keys_str_mv	AT rudrappathimmaraju peroxidaseproductionfromhairyrootculturesofredbeetbetavulgaris AT neelwarnebhagyalakshmi peroxidaseproductionfromhairyrootculturesofredbeetbetavulgaris AT kumarvinod peroxidaseproductionfromhairyrootculturesofredbeetbetavulgaris AT lakshmananvenkatachalam peroxidaseproductionfromhairyrootculturesofredbeetbetavulgaris AT venkataramareddysreedharreddampalli peroxidaseproductionfromhairyrootculturesofredbeetbetavulgaris AT aswathanarayanaravishankargokare peroxidaseproductionfromhairyrootculturesofredbeetbetavulgaris
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Peroxidase production from hairy root cultures of red beet (Beta vulgaris)

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