Wild ornamental germplasm exploration and domestication based on biotechnological approaches: In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis

The genus Scoparia is native from Argentina. The diversity of colours and shape of their flowers bestows this genus a very interesting ornamental potential. The purpose of the present study is to explore the Scoparia species germplasm by means of in vitro polyploidization in order to improve their o...

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Autores principales:	Escandón,Alejandro S, Miyajima,Ikuo, Alderete,Marisol, Hagiwara,Juan Carlos, Facciuto,Gabriela, Mata,Diego, Soto,Silvina M
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2005
Materias:	chimera flow cytometry micropropagation ornamental plants tetraploid
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200010
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spelling	oai:scielo:S0717-345820050002000102005-10-14Wild ornamental germplasm exploration and domestication based on biotechnological approaches: In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensisEscandón,Alejandro SMiyajima,IkuoAlderete,MarisolHagiwara,Juan CarlosFacciuto,GabrielaMata,DiegoSoto,Silvina M chimera flow cytometry micropropagation ornamental plants tetraploid The genus Scoparia is native from Argentina. The diversity of colours and shape of their flowers bestows this genus a very interesting ornamental potential. The purpose of the present study is to explore the Scoparia species germplasm by means of in vitro polyploidization in order to improve their ornamental qualities. Accessions of S. montevidiensis var. montevidiensis, S. montevidiensis var. glandulifera, S. nudicaulis, S. hasleriana and S. dulcis were collected and maintained under greenhouse conditions. The Murashige-Skoog medium, supplemented with 0.25 mg/L BAP was used for the nodal segments multiplication of the five Scoparia species. Except for S. hasleriana, the multiplication rate of the other species ranged between 10 and 12 shoots per explant. The colchicine doses tested with S. montevidiensis were: 0.0; 0.1; 0.05; 0.01 and 0.001% (24 and 48 hrs). From a total of 364 recovered plants, 4 solid tetraploid and 16 chimeras were detected. Significant differences were observed for the size of flower, leaves, and the stem diameter among the tetraploid plants and between them and the control. The tissue culture proved to be a powerful tool both to multiply the Scoparia material incorporated to our germplasm collection and to obtain new improved varieties of this beautiful genusinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.8 n.2 20052005-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200010en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	chimera flow cytometry micropropagation ornamental plants tetraploid
spellingShingle	chimera flow cytometry micropropagation ornamental plants tetraploid Escandón,Alejandro S Miyajima,Ikuo Alderete,Marisol Hagiwara,Juan Carlos Facciuto,Gabriela Mata,Diego Soto,Silvina M Wild ornamental germplasm exploration and domestication based on biotechnological approaches: In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis
description	The genus Scoparia is native from Argentina. The diversity of colours and shape of their flowers bestows this genus a very interesting ornamental potential. The purpose of the present study is to explore the Scoparia species germplasm by means of in vitro polyploidization in order to improve their ornamental qualities. Accessions of S. montevidiensis var. montevidiensis, S. montevidiensis var. glandulifera, S. nudicaulis, S. hasleriana and S. dulcis were collected and maintained under greenhouse conditions. The Murashige-Skoog medium, supplemented with 0.25 mg/L BAP was used for the nodal segments multiplication of the five Scoparia species. Except for S. hasleriana, the multiplication rate of the other species ranged between 10 and 12 shoots per explant. The colchicine doses tested with S. montevidiensis were: 0.0; 0.1; 0.05; 0.01 and 0.001% (24 and 48 hrs). From a total of 364 recovered plants, 4 solid tetraploid and 16 chimeras were detected. Significant differences were observed for the size of flower, leaves, and the stem diameter among the tetraploid plants and between them and the control. The tissue culture proved to be a powerful tool both to multiply the Scoparia material incorporated to our germplasm collection and to obtain new improved varieties of this beautiful genus
author	Escandón,Alejandro S Miyajima,Ikuo Alderete,Marisol Hagiwara,Juan Carlos Facciuto,Gabriela Mata,Diego Soto,Silvina M
author_facet	Escandón,Alejandro S Miyajima,Ikuo Alderete,Marisol Hagiwara,Juan Carlos Facciuto,Gabriela Mata,Diego Soto,Silvina M
author_sort	Escandón,Alejandro S
title	Wild ornamental germplasm exploration and domestication based on biotechnological approaches: In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis
title_short	Wild ornamental germplasm exploration and domestication based on biotechnological approaches: In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis
title_full	Wild ornamental germplasm exploration and domestication based on biotechnological approaches: In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis
title_fullStr	Wild ornamental germplasm exploration and domestication based on biotechnological approaches: In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis
title_full_unstemmed	Wild ornamental germplasm exploration and domestication based on biotechnological approaches: In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis
title_sort	wild ornamental germplasm exploration and domestication based on biotechnological approaches: in vitro colchicine treatment to obtain a new cultivar of scoparia montevidiensis
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200010
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Wild ornamental germplasm exploration and domestication based on biotechnological approaches: In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis

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