Growth and antibacterial activity of Lentinula edodes in liquid media supplemented with agricultural wastes

Antibacterial activity of Lentinula edodes against Bacillus subtilis was evaluated in cell-free filtrates obtained after growth in 14 different culture media. The highest B. subtilis growth inhibition was promoted by filtrates of growth media supplemented with rice bran, vermiculite or molasses. L....

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Autores principales:	Hiroko Hassegawa,Regina, Megumi Kasuya,Maria Catarina, Dantas Vanetti,Maria Cristina
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2005
Materias:	bacterial inhibition bioconversion mycelial growth mushroom shiitake
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200011
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spelling	oai:scielo:S0717-345820050002000112005-10-14Growth and antibacterial activity of Lentinula edodes in liquid media supplemented with agricultural wastesHiroko Hassegawa,ReginaMegumi Kasuya,Maria CatarinaDantas Vanetti,Maria Cristina bacterial inhibition bioconversion mycelial growth mushroom shiitake Antibacterial activity of Lentinula edodes against Bacillus subtilis was evaluated in cell-free filtrates obtained after growth in 14 different culture media. The highest B. subtilis growth inhibition was promoted by filtrates of growth media supplemented with rice bran, vermiculite or molasses. L. edodes dry mycelial biomass in liquid culture with 0.5% added rice bran was 3.2 mg/ml, after growth for 30 days at 25ºC without shaking, and 4.3 mg/ml under orbital shaking (150 rpm). However, antibacterial activity, detected between 20 and 24 days of incubation of stationary cultures, was absent in filtrates of aerated cultures. Temperatures of 20-25ºC enhanced both growth and antibacterial activity. Optimum pH for L. edodes mycelial growth was 3.0-3.5, while for production of antibacterial substance(s) it was 4.5. Our results indicated that incubation conditions that enhance mycelial growth are quite different from those necessary for production of antibacterial substance(s) by L. edodesinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.8 n.2 20052005-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200011en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bacterial inhibition bioconversion mycelial growth mushroom shiitake
spellingShingle	bacterial inhibition bioconversion mycelial growth mushroom shiitake Hiroko Hassegawa,Regina Megumi Kasuya,Maria Catarina Dantas Vanetti,Maria Cristina Growth and antibacterial activity of Lentinula edodes in liquid media supplemented with agricultural wastes
description	Antibacterial activity of Lentinula edodes against Bacillus subtilis was evaluated in cell-free filtrates obtained after growth in 14 different culture media. The highest B. subtilis growth inhibition was promoted by filtrates of growth media supplemented with rice bran, vermiculite or molasses. L. edodes dry mycelial biomass in liquid culture with 0.5% added rice bran was 3.2 mg/ml, after growth for 30 days at 25ºC without shaking, and 4.3 mg/ml under orbital shaking (150 rpm). However, antibacterial activity, detected between 20 and 24 days of incubation of stationary cultures, was absent in filtrates of aerated cultures. Temperatures of 20-25ºC enhanced both growth and antibacterial activity. Optimum pH for L. edodes mycelial growth was 3.0-3.5, while for production of antibacterial substance(s) it was 4.5. Our results indicated that incubation conditions that enhance mycelial growth are quite different from those necessary for production of antibacterial substance(s) by L. edodes
author	Hiroko Hassegawa,Regina Megumi Kasuya,Maria Catarina Dantas Vanetti,Maria Cristina
author_facet	Hiroko Hassegawa,Regina Megumi Kasuya,Maria Catarina Dantas Vanetti,Maria Cristina
author_sort	Hiroko Hassegawa,Regina
title	Growth and antibacterial activity of Lentinula edodes in liquid media supplemented with agricultural wastes
title_short	Growth and antibacterial activity of Lentinula edodes in liquid media supplemented with agricultural wastes
title_full	Growth and antibacterial activity of Lentinula edodes in liquid media supplemented with agricultural wastes
title_fullStr	Growth and antibacterial activity of Lentinula edodes in liquid media supplemented with agricultural wastes
title_full_unstemmed	Growth and antibacterial activity of Lentinula edodes in liquid media supplemented with agricultural wastes
title_sort	growth and antibacterial activity of lentinula edodes in liquid media supplemented with agricultural wastes
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200011
work_keys_str_mv	AT hirokohassegawaregina growthandantibacterialactivityoflentinulaedodesinliquidmediasupplementedwithagriculturalwastes AT megumikasuyamariacatarina growthandantibacterialactivityoflentinulaedodesinliquidmediasupplementedwithagriculturalwastes AT dantasvanettimariacristina growthandantibacterialactivityoflentinulaedodesinliquidmediasupplementedwithagriculturalwastes
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Growth and antibacterial activity of Lentinula edodes in liquid media supplemented with agricultural wastes

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