LaNe RAGE: a new tool for genomic DNA flanking sequence determination

The determination of genomic DNA sequence flanking a known region is often problematic. Existing technologies depend on multiple, efficient enzyme-catalysed preparative processing steps and/or rely on relatively inefficient one-sided' PCR mechanisms. I demonstrate the application of a simple ...

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Autor principal:	Park,Daniel J
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2005
Materias:	cloning DNA fingerprinting genomic DNA walking PCR sequencing transposon mutagenesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200012
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spelling	oai:scielo:S0717-345820050002000122005-10-14LaNe RAGE: a new tool for genomic DNA flanking sequence determinationPark,Daniel J cloning DNA fingerprinting genomic DNA walking PCR sequencing transposon mutagenesis The determination of genomic DNA sequence flanking a known region is often problematic. Existing technologies depend on multiple, efficient enzyme-catalysed preparative processing steps and/or rely on relatively inefficient one-sided' PCR mechanisms. I demonstrate the application of a simple two-sided' PCR-based approach, lariat-dependent nested PCR for rapid amplification of genomic DNA ends (LaNe RAGE), applied to the mouse GAPDH and PGK1 gene flanking sequences. This demonstration offers great promise in applications such as genome walking, transposon mutagenesis mapping and DNA fingerprintinginfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.8 n.2 20052005-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200012en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cloning DNA fingerprinting genomic DNA walking PCR sequencing transposon mutagenesis
spellingShingle	cloning DNA fingerprinting genomic DNA walking PCR sequencing transposon mutagenesis Park,Daniel J LaNe RAGE: a new tool for genomic DNA flanking sequence determination
description	The determination of genomic DNA sequence flanking a known region is often problematic. Existing technologies depend on multiple, efficient enzyme-catalysed preparative processing steps and/or rely on relatively inefficient one-sided' PCR mechanisms. I demonstrate the application of a simple two-sided' PCR-based approach, lariat-dependent nested PCR for rapid amplification of genomic DNA ends (LaNe RAGE), applied to the mouse GAPDH and PGK1 gene flanking sequences. This demonstration offers great promise in applications such as genome walking, transposon mutagenesis mapping and DNA fingerprinting
author	Park,Daniel J
author_facet	Park,Daniel J
author_sort	Park,Daniel J
title	LaNe RAGE: a new tool for genomic DNA flanking sequence determination
title_short	LaNe RAGE: a new tool for genomic DNA flanking sequence determination
title_full	LaNe RAGE: a new tool for genomic DNA flanking sequence determination
title_fullStr	LaNe RAGE: a new tool for genomic DNA flanking sequence determination
title_full_unstemmed	LaNe RAGE: a new tool for genomic DNA flanking sequence determination
title_sort	lane rage: a new tool for genomic dna flanking sequence determination
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200012
work_keys_str_mv	AT parkdanielj lanerageanewtoolforgenomicdnaflankingsequencedetermination
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LaNe RAGE: a new tool for genomic DNA flanking sequence determination

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