Effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of Grindelia pulchella

Grindelia pulchella callus and cell suspension cultures were established from seedling leaves. When several phytoregulator supplementations were assayed in solid Murashige and Skoog medium containing 3% (w/v) of sucrose (MS medium), combinations of indole-3-butyric acid (IBA) and N6-benzylaminopurin...

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Autores principales:	Hernandez,Xenia E, Orden,Alejandro A, Giordano,Oscar S, Kurina,Marcela
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2005
Materias:	Cu2+ dimethylsulfoxide permeabilization grindelane diterpenes jasmonic acid
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300007
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spelling	oai:scielo:S0717-345820050003000072006-01-26Effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of Grindelia pulchellaHernandez,Xenia EOrden,Alejandro AGiordano,Oscar SKurina,Marcela Cu2+ dimethylsulfoxide permeabilization grindelane diterpenes jasmonic acid Grindelia pulchella callus and cell suspension cultures were established from seedling leaves. When several phytoregulator supplementations were assayed in solid Murashige and Skoog medium containing 3% (w/v) of sucrose (MS medium), combinations of indole-3-butyric acid (IBA) and N6-benzylaminopurine (BA) resulted the most appropriate conditions to generate fast growing friable calli with detectable levels of grindelic acid. Moreover, the same basal media supplemented with 20.0 µM IBA/4.4 µM BA was found to be optimal for cell growth in submerged cultures (µmax = 0.26 days-1) while the addition of 20.0 µM IBA/18.0 µM BA resulted in a relative higher metabolite production (4.55 mg/gDW) when the inocula was 5% (v/v). Furthermore, three different stress factors and combinations of them were used to elicit cell suspensions. These experiments demonstrated that the combination of CuSO4 and dimethylsulfoxide (DMSO) increase the grindelic acid production to 2.63 mg/gDW in the elicited essay versus 0.756 mg/gDW in the control, at expense of cell growth. In contrast, the addition of jasmonic acid (JA) alone and combined with DMSO neither affected cell growth nor grindelic acid accumulationinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.8 n.3 20052005-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300007en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cu2+ dimethylsulfoxide permeabilization grindelane diterpenes jasmonic acid
spellingShingle	Cu2+ dimethylsulfoxide permeabilization grindelane diterpenes jasmonic acid Hernandez,Xenia E Orden,Alejandro A Giordano,Oscar S Kurina,Marcela Effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of Grindelia pulchella
description	Grindelia pulchella callus and cell suspension cultures were established from seedling leaves. When several phytoregulator supplementations were assayed in solid Murashige and Skoog medium containing 3% (w/v) of sucrose (MS medium), combinations of indole-3-butyric acid (IBA) and N6-benzylaminopurine (BA) resulted the most appropriate conditions to generate fast growing friable calli with detectable levels of grindelic acid. Moreover, the same basal media supplemented with 20.0 µM IBA/4.4 µM BA was found to be optimal for cell growth in submerged cultures (µmax = 0.26 days-1) while the addition of 20.0 µM IBA/18.0 µM BA resulted in a relative higher metabolite production (4.55 mg/gDW) when the inocula was 5% (v/v). Furthermore, three different stress factors and combinations of them were used to elicit cell suspensions. These experiments demonstrated that the combination of CuSO4 and dimethylsulfoxide (DMSO) increase the grindelic acid production to 2.63 mg/gDW in the elicited essay versus 0.756 mg/gDW in the control, at expense of cell growth. In contrast, the addition of jasmonic acid (JA) alone and combined with DMSO neither affected cell growth nor grindelic acid accumulation
author	Hernandez,Xenia E Orden,Alejandro A Giordano,Oscar S Kurina,Marcela
author_facet	Hernandez,Xenia E Orden,Alejandro A Giordano,Oscar S Kurina,Marcela
author_sort	Hernandez,Xenia E
title	Effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of Grindelia pulchella
title_short	Effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of Grindelia pulchella
title_full	Effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of Grindelia pulchella
title_fullStr	Effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of Grindelia pulchella
title_full_unstemmed	Effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of Grindelia pulchella
title_sort	effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of grindelia pulchella
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300007
work_keys_str_mv	AT hernandezxeniae effectsofelicitorandcoppersulfateongrindelicacidproductioninsubmergedculturesofgrindeliapulchella AT ordenalejandroa effectsofelicitorandcoppersulfateongrindelicacidproductioninsubmergedculturesofgrindeliapulchella AT giordanooscars effectsofelicitorandcoppersulfateongrindelicacidproductioninsubmergedculturesofgrindeliapulchella AT kurinamarcela effectsofelicitorandcoppersulfateongrindelicacidproductioninsubmergedculturesofgrindeliapulchella
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Effects of elicitor and copper sulfate on grindelic acid production in submerged cultures of Grindelia pulchella

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