Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants

Mutant strains from Aspergillus niger UAM-GS1 were produced by UV radiation to increase their hemicellulolytic and cellulolytic activity production. The mutant strains showing more enzymatic activity were those labelled GS1-S059 and GS1-S067. These strains also showed the largest relationship betwee...

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Autores principales:	De Nicolás-Santiago,Soledad, Regalado-González,Carlos, García-Almendárez,Blanca, Fernández,Francisco J, Téllez-Jurado,Alejandro, Huerta-Ochoa,Sergio
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	Aspergillus niger mutation mannanase production
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100008
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spelling	oai:scielo:S0717-345820060001000082006-05-11Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutantsDe Nicolás-Santiago,SoledadRegalado-González,CarlosGarcía-Almendárez,BlancaFernández,Francisco JTéllez-Jurado,AlejandroHuerta-Ochoa,Sergio Aspergillus niger mutation mannanase production Mutant strains from Aspergillus niger UAM-GS1 were produced by UV radiation to increase their hemicellulolytic and cellulolytic activity production. The mutant strains showing more enzymatic activity were those labelled GS1-S059 and GS1-S067. These strains also showed the largest relationship between diameter of hydrolysis zone and colony diameter. The mutant GS1-S067 showed a colony radial extension rate and a biomass growth rate g biomass/(cm² h), 1.17 times higher than that achieved by strain UAM-GS1. The high invasive capacity makes this mutant strain a promising alternative for its use in solid substrate fermentation (SSF). The morphological properties of the two mutant strains were evaluated by using scanning electron microscopy. The diameter of the sporangium of the mutant strains GS1-S059 and GS1-S067 was significantly larger (P < 0.05) than that found for the parental strain. The hypha length and diameter of the mutant strains significantly changed (P < 0.05) compared to the parental strain. A Pearson correlation analysis on hypha length, sporangium diameter, and cellulase and xylanase activities indicated that there was a strong relationship among these variables in relation to mannanase activity. Mutant strains GS1-S059 and GS1-S067 significantly increased their level of mannanase, xylanase and cellulase production, compared to the parental strain, improving their potential industrial applications.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.1 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100008en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aspergillus niger mutation mannanase production
spellingShingle	Aspergillus niger mutation mannanase production De Nicolás-Santiago,Soledad Regalado-González,Carlos García-Almendárez,Blanca Fernández,Francisco J Téllez-Jurado,Alejandro Huerta-Ochoa,Sergio Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants
description	Mutant strains from Aspergillus niger UAM-GS1 were produced by UV radiation to increase their hemicellulolytic and cellulolytic activity production. The mutant strains showing more enzymatic activity were those labelled GS1-S059 and GS1-S067. These strains also showed the largest relationship between diameter of hydrolysis zone and colony diameter. The mutant GS1-S067 showed a colony radial extension rate and a biomass growth rate g biomass/(cm² h), 1.17 times higher than that achieved by strain UAM-GS1. The high invasive capacity makes this mutant strain a promising alternative for its use in solid substrate fermentation (SSF). The morphological properties of the two mutant strains were evaluated by using scanning electron microscopy. The diameter of the sporangium of the mutant strains GS1-S059 and GS1-S067 was significantly larger (P < 0.05) than that found for the parental strain. The hypha length and diameter of the mutant strains significantly changed (P < 0.05) compared to the parental strain. A Pearson correlation analysis on hypha length, sporangium diameter, and cellulase and xylanase activities indicated that there was a strong relationship among these variables in relation to mannanase activity. Mutant strains GS1-S059 and GS1-S067 significantly increased their level of mannanase, xylanase and cellulase production, compared to the parental strain, improving their potential industrial applications.
author	De Nicolás-Santiago,Soledad Regalado-González,Carlos García-Almendárez,Blanca Fernández,Francisco J Téllez-Jurado,Alejandro Huerta-Ochoa,Sergio
author_facet	De Nicolás-Santiago,Soledad Regalado-González,Carlos García-Almendárez,Blanca Fernández,Francisco J Téllez-Jurado,Alejandro Huerta-Ochoa,Sergio
author_sort	De Nicolás-Santiago,Soledad
title	Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants
title_short	Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants
title_full	Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants
title_fullStr	Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants
title_full_unstemmed	Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants
title_sort	physiological, morphological, and mannanase production studies on aspergillus niger uam-gs1 mutants
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100008
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Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants

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