Specific nutrient supplementation of defined serum-free medium for the improvement of CHO cells growth and t-PA production

Recombinant CHO TF70R cells are able to grow and produce t-PA on serum-free medium BIOPRO1 (BioWhitaker Europe, Belgium). The purpose of the present study was to determine the effect of medium supplementation with vitamins, lipids, and specific amino acids on cell growth, t-PA production and biologi...

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Autores principales:	Altamirano,Claudia, Illanes,Andrés, Canessa,Rossana, Becerra,Silvana
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	amino acid cholesterol vitamins
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100009
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spelling	oai:scielo:S0717-345820060001000092006-05-11Specific nutrient supplementation of defined serum-free medium for the improvement of CHO cells growth and t-PA productionAltamirano,ClaudiaIllanes,AndrésCanessa,RossanaBecerra,Silvana amino acid cholesterol vitamins Recombinant CHO TF70R cells are able to grow and produce t-PA on serum-free medium BIOPRO1 (BioWhitaker Europe, Belgium). The purpose of the present study was to determine the effect of medium supplementation with vitamins, lipids, and specific amino acids on cell growth, t-PA production and biological functionality. Among vitamins, only biotin, folic acid, cobalamine and benzoic acid were required for improving growth and t-PA production. Lipid supplement allowed a significant increase cell concentration and t-PA specific activity and concentration, though its specific production rate decreased slightly. Medium supplementation with proline, serine and asparagine had also positive effects on cell growth. Besides, the addition of asparagine (even in the presence of glutamine) was essential for the production and biological quality of the t-PA. This systematic approach for media supplementation produced an increase in cell concentration around 100% and in t-PA production around 80%, with no detrimental effect on its biological activity. The effect of asparagine on t-PA production was unexpected and needs to be further studied. The above modifications of the production medium did not produce a significant effect on the metabolism of the main carbon and energy sources (glucose and glutamine) and the level of by-product formation (lactate and ammonia).info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.1 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100009en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	amino acid cholesterol vitamins
spellingShingle	amino acid cholesterol vitamins Altamirano,Claudia Illanes,Andrés Canessa,Rossana Becerra,Silvana Specific nutrient supplementation of defined serum-free medium for the improvement of CHO cells growth and t-PA production
description	Recombinant CHO TF70R cells are able to grow and produce t-PA on serum-free medium BIOPRO1 (BioWhitaker Europe, Belgium). The purpose of the present study was to determine the effect of medium supplementation with vitamins, lipids, and specific amino acids on cell growth, t-PA production and biological functionality. Among vitamins, only biotin, folic acid, cobalamine and benzoic acid were required for improving growth and t-PA production. Lipid supplement allowed a significant increase cell concentration and t-PA specific activity and concentration, though its specific production rate decreased slightly. Medium supplementation with proline, serine and asparagine had also positive effects on cell growth. Besides, the addition of asparagine (even in the presence of glutamine) was essential for the production and biological quality of the t-PA. This systematic approach for media supplementation produced an increase in cell concentration around 100% and in t-PA production around 80%, with no detrimental effect on its biological activity. The effect of asparagine on t-PA production was unexpected and needs to be further studied. The above modifications of the production medium did not produce a significant effect on the metabolism of the main carbon and energy sources (glucose and glutamine) and the level of by-product formation (lactate and ammonia).
author	Altamirano,Claudia Illanes,Andrés Canessa,Rossana Becerra,Silvana
author_facet	Altamirano,Claudia Illanes,Andrés Canessa,Rossana Becerra,Silvana
author_sort	Altamirano,Claudia
title	Specific nutrient supplementation of defined serum-free medium for the improvement of CHO cells growth and t-PA production
title_short	Specific nutrient supplementation of defined serum-free medium for the improvement of CHO cells growth and t-PA production
title_full	Specific nutrient supplementation of defined serum-free medium for the improvement of CHO cells growth and t-PA production
title_fullStr	Specific nutrient supplementation of defined serum-free medium for the improvement of CHO cells growth and t-PA production
title_full_unstemmed	Specific nutrient supplementation of defined serum-free medium for the improvement of CHO cells growth and t-PA production
title_sort	specific nutrient supplementation of defined serum-free medium for the improvement of cho cells growth and t-pa production
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100009
work_keys_str_mv	AT altamiranoclaudia specificnutrientsupplementationofdefinedserumfreemediumfortheimprovementofchocellsgrowthandtpaproduction AT illanesandres specificnutrientsupplementationofdefinedserumfreemediumfortheimprovementofchocellsgrowthandtpaproduction AT canessarossana specificnutrientsupplementationofdefinedserumfreemediumfortheimprovementofchocellsgrowthandtpaproduction AT becerrasilvana specificnutrientsupplementationofdefinedserumfreemediumfortheimprovementofchocellsgrowthandtpaproduction
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Specific nutrient supplementation of defined serum-free medium for the improvement of CHO cells growth and t-PA production

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