A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis

For studying genetic diversity in natural populations of Terminalia, a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to is...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sarwat,Maryam, Singh Negi,Madan, Lakshmikumaran,Malathi, Kumar Tyagi,Akhilesh, Das,Sandip, Shankar Srivastava,Prem
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	DNA extraction medicinal plants molecular markers Terminalia species
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100011
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582006000100011
record_format	dspace
spelling	oai:scielo:S0717-345820060001000112006-05-11A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysisSarwat,MaryamSingh Negi,MadanLakshmikumaran,MalathiKumar Tyagi,AkhileshDas,SandipShankar Srivastava,Prem DNA extraction medicinal plants molecular markers Terminalia species For studying genetic diversity in natural populations of Terminalia, a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to isolate DNA from Terminalia arjuna. The DNA isolated using this standardized protocol was high in quality and suitable for restriction digestion and generation of random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.1 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100011en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	DNA extraction medicinal plants molecular markers Terminalia species
spellingShingle	DNA extraction medicinal plants molecular markers Terminalia species Sarwat,Maryam Singh Negi,Madan Lakshmikumaran,Malathi Kumar Tyagi,Akhilesh Das,Sandip Shankar Srivastava,Prem A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis
description	For studying genetic diversity in natural populations of Terminalia, a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to isolate DNA from Terminalia arjuna. The DNA isolated using this standardized protocol was high in quality and suitable for restriction digestion and generation of random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).
author	Sarwat,Maryam Singh Negi,Madan Lakshmikumaran,Malathi Kumar Tyagi,Akhilesh Das,Sandip Shankar Srivastava,Prem
author_facet	Sarwat,Maryam Singh Negi,Madan Lakshmikumaran,Malathi Kumar Tyagi,Akhilesh Das,Sandip Shankar Srivastava,Prem
author_sort	Sarwat,Maryam
title	A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis
title_short	A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis
title_full	A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis
title_fullStr	A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis
title_full_unstemmed	A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis
title_sort	standardized protocol for genomic dna isolation from terminalia arjuna for genetic diversity analysis
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100011
work_keys_str_mv	AT sarwatmaryam astandardizedprotocolforgenomicdnaisolationfromterminaliaarjunaforgeneticdiversityanalysis AT singhnegimadan astandardizedprotocolforgenomicdnaisolationfromterminaliaarjunaforgeneticdiversityanalysis AT lakshmikumaranmalathi astandardizedprotocolforgenomicdnaisolationfromterminaliaarjunaforgeneticdiversityanalysis AT kumartyagiakhilesh astandardizedprotocolforgenomicdnaisolationfromterminaliaarjunaforgeneticdiversityanalysis AT dassandip astandardizedprotocolforgenomicdnaisolationfromterminaliaarjunaforgeneticdiversityanalysis AT shankarsrivastavaprem astandardizedprotocolforgenomicdnaisolationfromterminaliaarjunaforgeneticdiversityanalysis AT sarwatmaryam standardizedprotocolforgenomicdnaisolationfromterminaliaarjunaforgeneticdiversityanalysis AT singhnegimadan standardizedprotocolforgenomicdnaisolationfromterminaliaarjunaforgeneticdiversityanalysis AT lakshmikumaranmalathi standardizedprotocolforgenomicdnaisolationfromterminaliaarjunaforgeneticdiversityanalysis AT kumartyagiakhilesh standardizedprotocolforgenomicdnaisolationfromterminaliaarjunaforgeneticdiversityanalysis AT dassandip standardizedprotocolforgenomicdnaisolationfromterminaliaarjunaforgeneticdiversityanalysis AT shankarsrivastavaprem standardizedprotocolforgenomicdnaisolationfromterminaliaarjunaforgeneticdiversityanalysis
_version_	1718441740862488576

A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis

Ejemplares similares