Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm

Oil palm, like all other crops, is susceptible to attack from several insect pests causing significant reduction in productivity. In the past, cry genes from Bacillus thuringiensis have been reported to be effective in conferring resistance towards insect pests in crops such as corn and rice. One of...

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Autores principales:	Lee,Mei-Phing, Yeun,Li-Huey, Abdullah,Ruslan
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	CryIA(b) gene gene expression insect resistance oil palm transformation rapid detection system transgenic oil palm
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200005
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spelling	oai:scielo:S0717-345820060002000052006-12-11Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palmLee,Mei-PhingYeun,Li-HueyAbdullah,Ruslan CryIA(b) gene gene expression insect resistance oil palm transformation rapid detection system transgenic oil palm Oil palm, like all other crops, is susceptible to attack from several insect pests causing significant reduction in productivity. In the past, cry genes from Bacillus thuringiensis have been reported to be effective in conferring resistance towards insect pests in crops such as corn and rice. One of the advantages of these toxin proteins is their specificity towards certain harmful insects. A rapid and efficient method was developed for the transformation and evaluation of CryIA(b) expression in oil palm. A recombinant vector was introduced into immature embryos (IEs) of oil palm via the biolistic method. More than 700 putative transformed IEs from independent transformation events were generated. Transient transformation efficiency of 81-100 % was achieved. We found that pre-treatment of target tissues with phytohormones is essential for increasing the transformation efficiency. This finding could enable higher transformation rate in oil palm that was previously difficult to transform. PCR analysis further confirmed the presence of the CryIA(b) in the transformed tissues. Expression of CryIA(b) from PCR-positive samples was further confirmed using a rapid gene expression detection system. This novel and rapid detection system could serve as a good opportunity to analyze the impact of transgenes upon transfer to the new environment, especially for crops with long generation cycle, such as oil palm.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.2 20062006-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200005en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	CryIA(b) gene gene expression insect resistance oil palm transformation rapid detection system transgenic oil palm
spellingShingle	CryIA(b) gene gene expression insect resistance oil palm transformation rapid detection system transgenic oil palm Lee,Mei-Phing Yeun,Li-Huey Abdullah,Ruslan Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm
description	Oil palm, like all other crops, is susceptible to attack from several insect pests causing significant reduction in productivity. In the past, cry genes from Bacillus thuringiensis have been reported to be effective in conferring resistance towards insect pests in crops such as corn and rice. One of the advantages of these toxin proteins is their specificity towards certain harmful insects. A rapid and efficient method was developed for the transformation and evaluation of CryIA(b) expression in oil palm. A recombinant vector was introduced into immature embryos (IEs) of oil palm via the biolistic method. More than 700 putative transformed IEs from independent transformation events were generated. Transient transformation efficiency of 81-100 % was achieved. We found that pre-treatment of target tissues with phytohormones is essential for increasing the transformation efficiency. This finding could enable higher transformation rate in oil palm that was previously difficult to transform. PCR analysis further confirmed the presence of the CryIA(b) in the transformed tissues. Expression of CryIA(b) from PCR-positive samples was further confirmed using a rapid gene expression detection system. This novel and rapid detection system could serve as a good opportunity to analyze the impact of transgenes upon transfer to the new environment, especially for crops with long generation cycle, such as oil palm.
author	Lee,Mei-Phing Yeun,Li-Huey Abdullah,Ruslan
author_facet	Lee,Mei-Phing Yeun,Li-Huey Abdullah,Ruslan
author_sort	Lee,Mei-Phing
title	Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm
title_short	Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm
title_full	Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm
title_fullStr	Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm
title_full_unstemmed	Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm
title_sort	expression of bacillus thuringiensis insecticidal protein gene in transgenic oil palm
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200005
work_keys_str_mv	AT leemeiphing expressionofbacillusthuringiensisinsecticidalproteingeneintransgenicoilpalm AT yeunlihuey expressionofbacillusthuringiensisinsecticidalproteingeneintransgenicoilpalm AT abdullahruslan expressionofbacillusthuringiensisinsecticidalproteingeneintransgenicoilpalm
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Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm

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