Identification of phase relative genes in tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta)

Genes differentially expressed between tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis were isolated by suppression subtractive hybridization (SSH) and screened by dot-blot macro-arrays. Different expression profiles of selected clones based on the results of dot-...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ren,Xueying, Zhang,Xuecheng, Sui,Zhenghong
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	female gametophyte Gracilaria/Gracilariopsis lemaneiformis suppression subtractive hybridization tetrasporophyte Virtual Northern blot
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200006
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582006000200006
record_format	dspace
spelling	oai:scielo:S0717-345820060002000062006-12-10Identification of phase relative genes in tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta)Ren,XueyingZhang,XuechengSui,Zhenghong female gametophyte Gracilaria/Gracilariopsis lemaneiformis suppression subtractive hybridization tetrasporophyte Virtual Northern blot Genes differentially expressed between tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis were isolated by suppression subtractive hybridization (SSH) and screened by dot-blot macro-arrays. Different expression profiles of selected clones based on the results of dot-blot macro-arrays were verified using virtual Northern blots. Totally, 14 phase relative cDNAs had been isolated and sequence identified. Among them, seven cDNAs were respectively homologous to crucial metabolic enzymes, Rab GTPase, RP42 homolog, and two hypothetical proteins, while the rest did not have significant hits in the databases examined. The results of virtual Northern blots revealed that 11 cDNAs were differentially expressed between the two samples, including 7 genes up-regulated in tetrasporophytes, 1 expressed exclusively in tetrasporophytes and 3 up-regulated in female gametophytes. By densitometric analysis relative to GAPDH, 8 cDNAs increased 1.3-4.2 fold and 3 decreased about 0.4-0.7 fold in tetrasporophytes compared to female gametophytes. The present study provides the first insight into genes that may involve in phase differentiation in G. lemaneiformis.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.2 20062006-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200006en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	female gametophyte Gracilaria/Gracilariopsis lemaneiformis suppression subtractive hybridization tetrasporophyte Virtual Northern blot
spellingShingle	female gametophyte Gracilaria/Gracilariopsis lemaneiformis suppression subtractive hybridization tetrasporophyte Virtual Northern blot Ren,Xueying Zhang,Xuecheng Sui,Zhenghong Identification of phase relative genes in tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta)
description	Genes differentially expressed between tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis were isolated by suppression subtractive hybridization (SSH) and screened by dot-blot macro-arrays. Different expression profiles of selected clones based on the results of dot-blot macro-arrays were verified using virtual Northern blots. Totally, 14 phase relative cDNAs had been isolated and sequence identified. Among them, seven cDNAs were respectively homologous to crucial metabolic enzymes, Rab GTPase, RP42 homolog, and two hypothetical proteins, while the rest did not have significant hits in the databases examined. The results of virtual Northern blots revealed that 11 cDNAs were differentially expressed between the two samples, including 7 genes up-regulated in tetrasporophytes, 1 expressed exclusively in tetrasporophytes and 3 up-regulated in female gametophytes. By densitometric analysis relative to GAPDH, 8 cDNAs increased 1.3-4.2 fold and 3 decreased about 0.4-0.7 fold in tetrasporophytes compared to female gametophytes. The present study provides the first insight into genes that may involve in phase differentiation in G. lemaneiformis.
author	Ren,Xueying Zhang,Xuecheng Sui,Zhenghong
author_facet	Ren,Xueying Zhang,Xuecheng Sui,Zhenghong
author_sort	Ren,Xueying
title	Identification of phase relative genes in tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta)
title_short	Identification of phase relative genes in tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta)
title_full	Identification of phase relative genes in tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta)
title_fullStr	Identification of phase relative genes in tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta)
title_full_unstemmed	Identification of phase relative genes in tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta)
title_sort	identification of phase relative genes in tetrasporophytes and female gametophytes of gracilaria/gracilariopsis lemaneiformis (gracilariales, rhodophyta)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200006
work_keys_str_mv	AT renxueying identificationofphaserelativegenesintetrasporophytesandfemalegametophytesofgracilariagracilariopsislemaneiformisgracilarialesrhodophyta AT zhangxuecheng identificationofphaserelativegenesintetrasporophytesandfemalegametophytesofgracilariagracilariopsislemaneiformisgracilarialesrhodophyta AT suizhenghong identificationofphaserelativegenesintetrasporophytesandfemalegametophytesofgracilariagracilariopsislemaneiformisgracilarialesrhodophyta
_version_	1718441742576910336

Identification of phase relative genes in tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta)

Ejemplares similares