Production of polyclonal antisera to manganese superoxide dismutase expressed in downy mildew resistant pearl millet and its application for immunodiagnosis

Production of polyclonal antisera to manganese superoxide dismutase expressed in downy mildew resistant pearl millet and its application for immunodiagnosis

Level of superoxide dismutase (SOD) in the seedlings of downy mildew resistant pearl millet genotype (IP 18293) increased 3-fold upon inoculation with Sclerospora graminicola. Mn-SOD was purified from pearl millet by ion-exchange and gel filtration chromatography. Polyclonal antibody was raised in r...

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Autores principales: Urs Babitha,Mysore Pandurangaraj, Madhu,Devaiah, Sripathi P,Harishchandra, Shekar Shetty,Hunthrike
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2006
Materias:
DIBA
downy mildew
ELISA
immunofluorescence
manganese superoxide dismutase
pearl millet
Sclerospora graminicola
Western blot
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200007
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