Protective effect of an antimicrobial peptide from Mytilus edulis chilensis expressed in Nicotiana tabacum L.

A "defensin-like" antibacterial peptide from Mytilus edulis chilensis, was sub-cloned into a binary vector for expression in plant tissues. The resulting new clone was electroporated into A. tumefaciens to transform tobacco plants. The presence of the construct in transgenic tobacco lines...

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Autores principales:	Arenas,Gloria, Marshall,Sergio H., Espinoza,Valeria, Ramírez,Ingrid, Peña-Cortés,Hugo
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	antibacterial peptide disease resistance in vivo expression Nicotiana tabacum L. Pseudomonassyringae pv. syringae transgenic tobacco plants
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200008
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spelling	oai:scielo:S0717-345820060002000082006-12-10Protective effect of an antimicrobial peptide from Mytilus edulis chilensis expressed in Nicotiana tabacum L.Arenas,GloriaMarshall,Sergio H.Espinoza,ValeriaRamírez,IngridPeña-Cortés,Hugo antibacterial peptide disease resistance in vivo expression Nicotiana tabacum L. Pseudomonassyringae pv. syringae transgenic tobacco plants A "defensin-like" antibacterial peptide from Mytilus edulis chilensis, was sub-cloned into a binary vector for expression in plant tissues. The resulting new clone was electroporated into A. tumefaciens to transform tobacco plants. The presence of the construct in transgenic tobacco lines was demonstrated through RT-PCR, Northern and Western blots. Transformed positive plants were selected and grown for challenging. Tobacco leaves were infiltrated with Pseudomonassyringae pv. syringae and visual lesions determined at different times post-exposure. Of seven plants exposed, four gave variable protection up to seven days post-infection while one of them appears to be fully protected. These results suggest that defensin-like antimicrobial peptides from molluscs are a good source to provide resistance of tobacco plants to Pseudomonassyringae pv. syringae.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.2 20062006-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200008en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	antibacterial peptide disease resistance in vivo expression Nicotiana tabacum L. Pseudomonassyringae pv. syringae transgenic tobacco plants
spellingShingle	antibacterial peptide disease resistance in vivo expression Nicotiana tabacum L. Pseudomonassyringae pv. syringae transgenic tobacco plants Arenas,Gloria Marshall,Sergio H. Espinoza,Valeria Ramírez,Ingrid Peña-Cortés,Hugo Protective effect of an antimicrobial peptide from Mytilus edulis chilensis expressed in Nicotiana tabacum L.
description	A "defensin-like" antibacterial peptide from Mytilus edulis chilensis, was sub-cloned into a binary vector for expression in plant tissues. The resulting new clone was electroporated into A. tumefaciens to transform tobacco plants. The presence of the construct in transgenic tobacco lines was demonstrated through RT-PCR, Northern and Western blots. Transformed positive plants were selected and grown for challenging. Tobacco leaves were infiltrated with Pseudomonassyringae pv. syringae and visual lesions determined at different times post-exposure. Of seven plants exposed, four gave variable protection up to seven days post-infection while one of them appears to be fully protected. These results suggest that defensin-like antimicrobial peptides from molluscs are a good source to provide resistance of tobacco plants to Pseudomonassyringae pv. syringae.
author	Arenas,Gloria Marshall,Sergio H. Espinoza,Valeria Ramírez,Ingrid Peña-Cortés,Hugo
author_facet	Arenas,Gloria Marshall,Sergio H. Espinoza,Valeria Ramírez,Ingrid Peña-Cortés,Hugo
author_sort	Arenas,Gloria
title	Protective effect of an antimicrobial peptide from Mytilus edulis chilensis expressed in Nicotiana tabacum L.
title_short	Protective effect of an antimicrobial peptide from Mytilus edulis chilensis expressed in Nicotiana tabacum L.
title_full	Protective effect of an antimicrobial peptide from Mytilus edulis chilensis expressed in Nicotiana tabacum L.
title_fullStr	Protective effect of an antimicrobial peptide from Mytilus edulis chilensis expressed in Nicotiana tabacum L.
title_full_unstemmed	Protective effect of an antimicrobial peptide from Mytilus edulis chilensis expressed in Nicotiana tabacum L.
title_sort	protective effect of an antimicrobial peptide from mytilus edulis chilensis expressed in nicotiana tabacum l.
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200008
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Protective effect of an antimicrobial peptide from Mytilus edulis chilensis expressed in Nicotiana tabacum L.

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