A new variety of Bacopa monnieri obtained by in vitro polyploidization

The commercial value of Bacopa monnieri, a widespread herbaceous plant in Argentina, can be substantially improved increasing its flower size by chromosome doubling with colchicine. MS supplemented with 0.25 mg/L of 6-benzylaminopurine proved to be an appropriate medium for the in vitro multiplicati...

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Autores principales:	Salvio Escandón,Alejandro, Hagiwara,Juan Carlos, Alderete,Liliana Marisol
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	colchicine flow cytometry ornamental plants Scrophulariaceae tetraploid
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300002
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spelling	oai:scielo:S0717-345820060003000022007-01-17A new variety of Bacopa monnieri obtained by in vitro polyploidizationSalvio Escandón,AlejandroHagiwara,Juan CarlosAlderete,Liliana Marisol colchicine flow cytometry ornamental plants Scrophulariaceae tetraploid The commercial value of Bacopa monnieri, a widespread herbaceous plant in Argentina, can be substantially improved increasing its flower size by chromosome doubling with colchicine. MS supplemented with 0.25 mg/L of 6-benzylaminopurine proved to be an appropriate medium for the in vitro multiplication of nodal segments of B. monnieri. Polyploidization was achieved submerging nodal segments during 24 or 48 hrs in colchicine solution 0.001 and 0.01% P/V, in 1% DMSO. Segments submerged in water and in DMSO 1% aqueous solution were used as controls. DNA contents from recovered plants was measured and characterized and their phenotype described. Two tetraploid plants originated from independent events were detected. These plants showed significant differences in size and colour both in leaves and flowers compared to untreated controls.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.3 20062006-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300002en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	colchicine flow cytometry ornamental plants Scrophulariaceae tetraploid
spellingShingle	colchicine flow cytometry ornamental plants Scrophulariaceae tetraploid Salvio Escandón,Alejandro Hagiwara,Juan Carlos Alderete,Liliana Marisol A new variety of Bacopa monnieri obtained by in vitro polyploidization
description	The commercial value of Bacopa monnieri, a widespread herbaceous plant in Argentina, can be substantially improved increasing its flower size by chromosome doubling with colchicine. MS supplemented with 0.25 mg/L of 6-benzylaminopurine proved to be an appropriate medium for the in vitro multiplication of nodal segments of B. monnieri. Polyploidization was achieved submerging nodal segments during 24 or 48 hrs in colchicine solution 0.001 and 0.01% P/V, in 1% DMSO. Segments submerged in water and in DMSO 1% aqueous solution were used as controls. DNA contents from recovered plants was measured and characterized and their phenotype described. Two tetraploid plants originated from independent events were detected. These plants showed significant differences in size and colour both in leaves and flowers compared to untreated controls.
author	Salvio Escandón,Alejandro Hagiwara,Juan Carlos Alderete,Liliana Marisol
author_facet	Salvio Escandón,Alejandro Hagiwara,Juan Carlos Alderete,Liliana Marisol
author_sort	Salvio Escandón,Alejandro
title	A new variety of Bacopa monnieri obtained by in vitro polyploidization
title_short	A new variety of Bacopa monnieri obtained by in vitro polyploidization
title_full	A new variety of Bacopa monnieri obtained by in vitro polyploidization
title_fullStr	A new variety of Bacopa monnieri obtained by in vitro polyploidization
title_full_unstemmed	A new variety of Bacopa monnieri obtained by in vitro polyploidization
title_sort	new variety of bacopa monnieri obtained by in vitro polyploidization
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300002
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A new variety of Bacopa monnieri obtained by in vitro polyploidization

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