Analysis of the molecular basis of Xanthomonas axonopodis pv. citri pathogenesis in Citrus limon

Xanthomonas axonopodis pathovar citri (Xac) causes bacterial citrus canker, a serious disease of most citrus species. Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot disease in cruciferous plants. In Xcc, cell-cell signaling is mediated by diffusible signal factor (DSF)....

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Autores principales:	Siciliano,Florencia, Torres,Pablo, Sendín,Lorena, Bermejo,Carolina, Filippone,Paula, Vellice,Gabriel, Ramallo,Jackie, Castagnaro,Atilio, Vojnov,Adrian, Marano,María Rosa
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	canker DSF extracellular polysaccharide quorum sensing
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300005
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spelling	oai:scielo:S0717-345820060003000052007-01-17Analysis of the molecular basis of Xanthomonas axonopodis pv. citri pathogenesis in Citrus limonSiciliano,FlorenciaTorres,PabloSendín,LorenaBermejo,CarolinaFilippone,PaulaVellice,GabrielRamallo,JackieCastagnaro,AtilioVojnov,AdrianMarano,María Rosa canker DSF extracellular polysaccharide quorum sensing Xanthomonas axonopodis pathovar citri (Xac) causes bacterial citrus canker, a serious disease of most citrus species. Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot disease in cruciferous plants. In Xcc, cell-cell signaling is mediated by diffusible signal factor (DSF). Synthesis of DSF depends on RpfB and RpfF. DSF perception and signal transduction have been suggested to involve a two-component system comprising RpfC and RpfG. It has been proposed that these proteins participate in a signal transduction system linking changes in the environment to the synthesis of DSF and the expression of virulence genes. Although the cluster of the rpf genes in Xac has synteny with the corresponding cluster in Xcc, two genes (rpfH and rpfI) are absent in Xac. To investigate DSF-mediated regulation during Xac-Citrus limon interaction, we constructed two strains of Xac, one with a mutation in the rpfF gene, leading to an inability to produce DSF, and one with a mutation in the rpfC gene leading to an overproduction of DSF. These mutants also show decreased levels of extracellular cyclic β-(1,2)-glucans and decreased production of endoglucanase and protease extracellular enzymes. The Xac DSF-deficient rpfF and the DSF-hyper producing rpfC mutants are both severely compromised in their ability to cause canker symptoms in lemon leaves compared to the wild-type. Here we provide evidence that rpf genes in Xac are involved in controlling virulence factors mediated by DSF.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.3 20062006-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300005en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	canker DSF extracellular polysaccharide quorum sensing
spellingShingle	canker DSF extracellular polysaccharide quorum sensing Siciliano,Florencia Torres,Pablo Sendín,Lorena Bermejo,Carolina Filippone,Paula Vellice,Gabriel Ramallo,Jackie Castagnaro,Atilio Vojnov,Adrian Marano,María Rosa Analysis of the molecular basis of Xanthomonas axonopodis pv. citri pathogenesis in Citrus limon
description	Xanthomonas axonopodis pathovar citri (Xac) causes bacterial citrus canker, a serious disease of most citrus species. Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot disease in cruciferous plants. In Xcc, cell-cell signaling is mediated by diffusible signal factor (DSF). Synthesis of DSF depends on RpfB and RpfF. DSF perception and signal transduction have been suggested to involve a two-component system comprising RpfC and RpfG. It has been proposed that these proteins participate in a signal transduction system linking changes in the environment to the synthesis of DSF and the expression of virulence genes. Although the cluster of the rpf genes in Xac has synteny with the corresponding cluster in Xcc, two genes (rpfH and rpfI) are absent in Xac. To investigate DSF-mediated regulation during Xac-Citrus limon interaction, we constructed two strains of Xac, one with a mutation in the rpfF gene, leading to an inability to produce DSF, and one with a mutation in the rpfC gene leading to an overproduction of DSF. These mutants also show decreased levels of extracellular cyclic β-(1,2)-glucans and decreased production of endoglucanase and protease extracellular enzymes. The Xac DSF-deficient rpfF and the DSF-hyper producing rpfC mutants are both severely compromised in their ability to cause canker symptoms in lemon leaves compared to the wild-type. Here we provide evidence that rpf genes in Xac are involved in controlling virulence factors mediated by DSF.
author	Siciliano,Florencia Torres,Pablo Sendín,Lorena Bermejo,Carolina Filippone,Paula Vellice,Gabriel Ramallo,Jackie Castagnaro,Atilio Vojnov,Adrian Marano,María Rosa
author_facet	Siciliano,Florencia Torres,Pablo Sendín,Lorena Bermejo,Carolina Filippone,Paula Vellice,Gabriel Ramallo,Jackie Castagnaro,Atilio Vojnov,Adrian Marano,María Rosa
author_sort	Siciliano,Florencia
title	Analysis of the molecular basis of Xanthomonas axonopodis pv. citri pathogenesis in Citrus limon
title_short	Analysis of the molecular basis of Xanthomonas axonopodis pv. citri pathogenesis in Citrus limon
title_full	Analysis of the molecular basis of Xanthomonas axonopodis pv. citri pathogenesis in Citrus limon
title_fullStr	Analysis of the molecular basis of Xanthomonas axonopodis pv. citri pathogenesis in Citrus limon
title_full_unstemmed	Analysis of the molecular basis of Xanthomonas axonopodis pv. citri pathogenesis in Citrus limon
title_sort	analysis of the molecular basis of xanthomonas axonopodis pv. citri pathogenesis in citrus limon
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300005
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Analysis of the molecular basis of Xanthomonas axonopodis pv. citri pathogenesis in Citrus limon

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