Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases

The objective of this work was the optimization of the conditions of in vitro culture for callus production of Silybum marianum (L.) Gaertn. (Asteraceae). Sections of cotyledons, previously disinfected by washing successively with ethanol 70º, NaClO (10% w/v) and Tween 20 (0.05% v/v) and ri...

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Autores principales:	Cimino,Cecilia, Vairo Cavalli,Sandra, Spina,Francisco, Natalucci,Claudia, Priolo,Nora
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	Asteraceae callus rhizogenesis Silybum marianum
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300012
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spelling	oai:scielo:S0717-345820060003000122007-01-17Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidasesCimino,CeciliaVairo Cavalli,SandraSpina,FranciscoNatalucci,ClaudiaPriolo,Nora Asteraceae callus rhizogenesis Silybum marianum The objective of this work was the optimization of the conditions of in vitro culture for callus production of Silybum marianum (L.) Gaertn. (Asteraceae). Sections of cotyledons, previously disinfected by washing successively with ethanol 70º, NaClO (10% w/v) and Tween 20 (0.05% v/v) and rinsing with sterile distilled water, were used as explants. For its initial culture, B5 medium supplemented with BA and 2,4-D solidified with phytagel was used, and a 63% survival was achieved. To obtain callus, two solid media were assayed (S1 and S2) using B5 medium supplemented with growth regulators (BA and 2,4-D or NAA and BA, respectively). The calli were grown at 25ºC during 45 days in darkness. Growth kinetics was studied using S1 medium obtaining a typical growth curve with an exponential phase after 14 days of incubation (rate of growth 0.005 g dry weight/ day) and stationary phase after 35 days. The rate of growth in S2 medium was slower, and rhizogenesis was observed starting on the fifth week of incubation. From these results, the best culture medium for callus production of Silybum marianum was S1 medium.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.3 20062006-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300012en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Asteraceae callus rhizogenesis Silybum marianum
spellingShingle	Asteraceae callus rhizogenesis Silybum marianum Cimino,Cecilia Vairo Cavalli,Sandra Spina,Francisco Natalucci,Claudia Priolo,Nora Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
description	The objective of this work was the optimization of the conditions of in vitro culture for callus production of Silybum marianum (L.) Gaertn. (Asteraceae). Sections of cotyledons, previously disinfected by washing successively with ethanol 70º, NaClO (10% w/v) and Tween 20 (0.05% v/v) and rinsing with sterile distilled water, were used as explants. For its initial culture, B5 medium supplemented with BA and 2,4-D solidified with phytagel was used, and a 63% survival was achieved. To obtain callus, two solid media were assayed (S1 and S2) using B5 medium supplemented with growth regulators (BA and 2,4-D or NAA and BA, respectively). The calli were grown at 25ºC during 45 days in darkness. Growth kinetics was studied using S1 medium obtaining a typical growth curve with an exponential phase after 14 days of incubation (rate of growth 0.005 g dry weight/ day) and stationary phase after 35 days. The rate of growth in S2 medium was slower, and rhizogenesis was observed starting on the fifth week of incubation. From these results, the best culture medium for callus production of Silybum marianum was S1 medium.
author	Cimino,Cecilia Vairo Cavalli,Sandra Spina,Francisco Natalucci,Claudia Priolo,Nora
author_facet	Cimino,Cecilia Vairo Cavalli,Sandra Spina,Francisco Natalucci,Claudia Priolo,Nora
author_sort	Cimino,Cecilia
title	Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
title_short	Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
title_full	Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
title_fullStr	Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
title_full_unstemmed	Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
title_sort	callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300012
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Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases

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