Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites
The objective of the present work was to establish the molecular identification profile for six new varieties of Nierembergia linariaefolia to incorporate the fingerprint, as complementary information to the standard registration data. Total DNA was extracted from young leaves following the protocol...
Guardado en:
| Autores principales: | , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Pontificia Universidad Católica de Valparaíso
2006
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300014 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0717-34582006000300014 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0717-345820060003000142007-01-17Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellitesPérez de la Torre,Mariana C.Escandón,Alejandro S. ISSR ornamentals varietal identification The objective of the present work was to establish the molecular identification profile for six new varieties of Nierembergia linariaefolia to incorporate the fingerprint, as complementary information to the standard registration data. Total DNA was extracted from young leaves following the protocol of the cetyltrimethylammonium bromide. Anchored microsatellites were used as molecular markers. The amplification reactions were carried out with seven primers. A total of 251 loci were detected, 98% of them were polymorphic. The average of polymorphic loci was 35 loci per primer and, 41 loci per genotype. Six out of the seven primers used discriminated all the individuals involved in the present study; consequently, it was possible to generate the molecular identification profile for the six new varieties. This result, supported together with our previous reports, indicates that the anchored microsatellites are a very useful technique for the fingerprints generation in N. linariaefolia.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.3 20062006-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300014en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
ISSR ornamentals varietal identification |
| spellingShingle |
ISSR ornamentals varietal identification Pérez de la Torre,Mariana C. Escandón,Alejandro S. Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites |
| description |
The objective of the present work was to establish the molecular identification profile for six new varieties of Nierembergia linariaefolia to incorporate the fingerprint, as complementary information to the standard registration data. Total DNA was extracted from young leaves following the protocol of the cetyltrimethylammonium bromide. Anchored microsatellites were used as molecular markers. The amplification reactions were carried out with seven primers. A total of 251 loci were detected, 98% of them were polymorphic. The average of polymorphic loci was 35 loci per primer and, 41 loci per genotype. Six out of the seven primers used discriminated all the individuals involved in the present study; consequently, it was possible to generate the molecular identification profile for the six new varieties. This result, supported together with our previous reports, indicates that the anchored microsatellites are a very useful technique for the fingerprints generation in N. linariaefolia. |
| author |
Pérez de la Torre,Mariana C. Escandón,Alejandro S. |
| author_facet |
Pérez de la Torre,Mariana C. Escandón,Alejandro S. |
| author_sort |
Pérez de la Torre,Mariana C. |
| title |
Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites |
| title_short |
Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites |
| title_full |
Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites |
| title_fullStr |
Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites |
| title_full_unstemmed |
Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites |
| title_sort |
construction of a molecular identification profile of new varieties of nierembergia linariaefolia by anchored microsatellites |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2006 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300014 |
| work_keys_str_mv |
AT perezdelatorremarianac constructionofamolecularidentificationprofileofnewvarietiesofnierembergialinariaefoliabyanchoredmicrosatellites AT escandonalejandros constructionofamolecularidentificationprofileofnewvarietiesofnierembergialinariaefoliabyanchoredmicrosatellites |
| _version_ |
1718441747970785280 |