Establishment of an in vitro micropropagation protocol for Mecardonia tenella

Mecardonia tenella is an attractive herbaceous native plant, characterized by their intense green foliage and their abundant yellow flowers. It is a very interesting plant for pot and/or garden. A trait to improve in this species is the size of the flowers. The goal of the present paper is to study...

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Autores principales:	Alderete,Liliana Marisol, Mori,Marcela, Kato,Adriana, Salvio Escandón,Alejandro
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	in vitro micropropagation Scrophulariaceae
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300017
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spelling	oai:scielo:S0717-345820060003000172007-01-17Establishment of an in vitro micropropagation protocol for Mecardonia tenellaAlderete,Liliana MarisolMori,MarcelaKato,AdrianaSalvio Escandón,Alejandro in vitro micropropagation Scrophulariaceae Mecardonia tenella is an attractive herbaceous native plant, characterized by their intense green foliage and their abundant yellow flowers. It is a very interesting plant for pot and/or garden. A trait to improve in this species is the size of the flowers. The goal of the present paper is to study the in vitro behaviour of M. tenella and to establish a routine protocol for its micropropagation. For the in vitro establishment of M. tenella, nodal segments were disinfected by standard methods using ethanol/sodium hypochloride and cultured on hormone-free MS medium, supplemented with a mixture of antibiotics and an antifungal. In order to study the hormonal requirements of the species, nodal segments were cultured on basal MS supplemented with antibiotic/antifungal mixture and the following concentrations of BAP and NAA (mg/L): 0.0; 0.25; 0.5 and 1.0. These plant regulators were tested in all possible combinations. In vitro plants growing in hormone-free medium were used as explant source. The best results were obtained in the treatments containing 0.25 and 0.5 mg/l BAP with a multiplication rate of 32 shoots per explant. The regenerated shoots rooted spontaneously. When transferred to the greenhouse, the ex vitro plants grew and flowered normally.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.3 20062006-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300017en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	in vitro micropropagation Scrophulariaceae
spellingShingle	in vitro micropropagation Scrophulariaceae Alderete,Liliana Marisol Mori,Marcela Kato,Adriana Salvio Escandón,Alejandro Establishment of an in vitro micropropagation protocol for Mecardonia tenella
description	Mecardonia tenella is an attractive herbaceous native plant, characterized by their intense green foliage and their abundant yellow flowers. It is a very interesting plant for pot and/or garden. A trait to improve in this species is the size of the flowers. The goal of the present paper is to study the in vitro behaviour of M. tenella and to establish a routine protocol for its micropropagation. For the in vitro establishment of M. tenella, nodal segments were disinfected by standard methods using ethanol/sodium hypochloride and cultured on hormone-free MS medium, supplemented with a mixture of antibiotics and an antifungal. In order to study the hormonal requirements of the species, nodal segments were cultured on basal MS supplemented with antibiotic/antifungal mixture and the following concentrations of BAP and NAA (mg/L): 0.0; 0.25; 0.5 and 1.0. These plant regulators were tested in all possible combinations. In vitro plants growing in hormone-free medium were used as explant source. The best results were obtained in the treatments containing 0.25 and 0.5 mg/l BAP with a multiplication rate of 32 shoots per explant. The regenerated shoots rooted spontaneously. When transferred to the greenhouse, the ex vitro plants grew and flowered normally.
author	Alderete,Liliana Marisol Mori,Marcela Kato,Adriana Salvio Escandón,Alejandro
author_facet	Alderete,Liliana Marisol Mori,Marcela Kato,Adriana Salvio Escandón,Alejandro
author_sort	Alderete,Liliana Marisol
title	Establishment of an in vitro micropropagation protocol for Mecardonia tenella
title_short	Establishment of an in vitro micropropagation protocol for Mecardonia tenella
title_full	Establishment of an in vitro micropropagation protocol for Mecardonia tenella
title_fullStr	Establishment of an in vitro micropropagation protocol for Mecardonia tenella
title_full_unstemmed	Establishment of an in vitro micropropagation protocol for Mecardonia tenella
title_sort	establishment of an in vitro micropropagation protocol for mecardonia tenella
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300017
work_keys_str_mv	AT alderetelilianamarisol establishmentofaninvitromicropropagationprotocolformecardoniatenella AT morimarcela establishmentofaninvitromicropropagationprotocolformecardoniatenella AT katoadriana establishmentofaninvitromicropropagationprotocolformecardoniatenella AT salvioescandonalejandro establishmentofaninvitromicropropagationprotocolformecardoniatenella
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Establishment of an in vitro micropropagation protocol for Mecardonia tenella

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