In vitro culture response of barley (Hordeum vulgare) ethylene synthesis mutant MC 169

Although it is generally accepted that plant in vitro culture response is influenced by the donor genotype, the genetic and molecular bases of this phenomenon are barely known. As a consequence, the optimization of tissue culture protocols is mainly empirically done. Researchers of the IGEAF studied...

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Autores principales:	Biagioli,Carolina, Rios,Raúl, Basso,Alicia, Franzone,Pascual, Prina,Alberto
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	callus induction ethylene habituated calli hydroponic culture regeneration
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300019
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spelling	oai:scielo:S0717-345820060003000192007-01-17In vitro culture response of barley (Hordeum vulgare) ethylene synthesis mutant MC 169Biagioli,CarolinaRios,RaúlBasso,AliciaFranzone,PascualPrina,Alberto callus induction ethylene habituated calli hydroponic culture regeneration Although it is generally accepted that plant in vitro culture response is influenced by the donor genotype, the genetic and molecular bases of this phenomenon are barely known. As a consequence, the optimization of tissue culture protocols is mainly empirically done. Researchers of the IGEAF studied the genetic basis of the in vitro regeneration of various plant species, including the tissue culture response of artificially induced barley mutants. One barley mutant, MC 169, carries a nuclear gene, recently described controlling the root growth in hydroponic cultivation. Under this condition, the roots of MC 169 mutant plants were longer than those of the original wild type line MC 182, a fact that was associated with a reduced ethylene biosynthesis. On the other hand, it is known that ethylene accumulation is inhibitory for in vitro regeneration of several plant species. In this study, we compared the in vitro culture response of mutant MC 169 with that of its mother line MC 182. The data about induction and regeneration of calli as well as those of habituated calli formation demonstrated that mutant MC 169 and its mother line MC 182 show a similar in vitro behaviour.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.3 20062006-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300019en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	callus induction ethylene habituated calli hydroponic culture regeneration
spellingShingle	callus induction ethylene habituated calli hydroponic culture regeneration Biagioli,Carolina Rios,Raúl Basso,Alicia Franzone,Pascual Prina,Alberto In vitro culture response of barley (Hordeum vulgare) ethylene synthesis mutant MC 169
description	Although it is generally accepted that plant in vitro culture response is influenced by the donor genotype, the genetic and molecular bases of this phenomenon are barely known. As a consequence, the optimization of tissue culture protocols is mainly empirically done. Researchers of the IGEAF studied the genetic basis of the in vitro regeneration of various plant species, including the tissue culture response of artificially induced barley mutants. One barley mutant, MC 169, carries a nuclear gene, recently described controlling the root growth in hydroponic cultivation. Under this condition, the roots of MC 169 mutant plants were longer than those of the original wild type line MC 182, a fact that was associated with a reduced ethylene biosynthesis. On the other hand, it is known that ethylene accumulation is inhibitory for in vitro regeneration of several plant species. In this study, we compared the in vitro culture response of mutant MC 169 with that of its mother line MC 182. The data about induction and regeneration of calli as well as those of habituated calli formation demonstrated that mutant MC 169 and its mother line MC 182 show a similar in vitro behaviour.
author	Biagioli,Carolina Rios,Raúl Basso,Alicia Franzone,Pascual Prina,Alberto
author_facet	Biagioli,Carolina Rios,Raúl Basso,Alicia Franzone,Pascual Prina,Alberto
author_sort	Biagioli,Carolina
title	In vitro culture response of barley (Hordeum vulgare) ethylene synthesis mutant MC 169
title_short	In vitro culture response of barley (Hordeum vulgare) ethylene synthesis mutant MC 169
title_full	In vitro culture response of barley (Hordeum vulgare) ethylene synthesis mutant MC 169
title_fullStr	In vitro culture response of barley (Hordeum vulgare) ethylene synthesis mutant MC 169
title_full_unstemmed	In vitro culture response of barley (Hordeum vulgare) ethylene synthesis mutant MC 169
title_sort	in vitro culture response of barley (hordeum vulgare) ethylene synthesis mutant mc 169
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300019
work_keys_str_mv	AT biagiolicarolina invitrocultureresponseofbarleyhordeumvulgareethylenesynthesismutantmc169 AT riosraul invitrocultureresponseofbarleyhordeumvulgareethylenesynthesismutantmc169 AT bassoalicia invitrocultureresponseofbarleyhordeumvulgareethylenesynthesismutantmc169 AT franzonepascual invitrocultureresponseofbarleyhordeumvulgareethylenesynthesismutantmc169 AT prinaalberto invitrocultureresponseofbarleyhordeumvulgareethylenesynthesismutantmc169
_version_	1718441749150433280

In vitro culture response of barley (Hordeum vulgare) ethylene synthesis mutant MC 169

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