Production of recombinant enzymes of wide use for research

For biotechnological purposes, protein expression refers to the directed synthesis of large amounts of desired proteins. The aim of the present work was to produce reverse transcriptase Moloney murine Leukaemia Virus retro-transcriptase and Taq DNA polymerase, as bioactive products. In the present p...

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Autores principales:	Manzur,María J., Muñoz,Rosana V., Lucero,Adrián A., Juri Ayub,Maximiliano, Alvarez,Sergio E., Ciuffo,Gladys M.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	bioactivity protein expression purification recombinant enzymes
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300023
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spelling	oai:scielo:S0717-345820060003000232007-01-17Production of recombinant enzymes of wide use for researchManzur,María J.Muñoz,Rosana V.Lucero,Adrián A.Juri Ayub,MaximilianoAlvarez,Sergio E.Ciuffo,Gladys M. bioactivity protein expression purification recombinant enzymes For biotechnological purposes, protein expression refers to the directed synthesis of large amounts of desired proteins. The aim of the present work was to produce reverse transcriptase Moloney murine Leukaemia Virus retro-transcriptase and Taq DNA polymerase, as bioactive products. In the present paper, we report the preparation of recombinant enzymes, expressed in E. coli strains. The enzymes produced exhibited quite good activity, compared with commercial enzymes, allowing us to replace the last ones for several lab applications. We are reporting changes and modifications to standard protocols described. The standard protocols were modified, i.e. for the purification step of Taq, a temperature dependent procedure was designed. The enzymes produced were used in different applications, such as PCR, RT-PCR, PCR Multiplex and RAPDs molecular markers.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.3 20062006-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300023en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bioactivity protein expression purification recombinant enzymes
spellingShingle	bioactivity protein expression purification recombinant enzymes Manzur,María J. Muñoz,Rosana V. Lucero,Adrián A. Juri Ayub,Maximiliano Alvarez,Sergio E. Ciuffo,Gladys M. Production of recombinant enzymes of wide use for research
description	For biotechnological purposes, protein expression refers to the directed synthesis of large amounts of desired proteins. The aim of the present work was to produce reverse transcriptase Moloney murine Leukaemia Virus retro-transcriptase and Taq DNA polymerase, as bioactive products. In the present paper, we report the preparation of recombinant enzymes, expressed in E. coli strains. The enzymes produced exhibited quite good activity, compared with commercial enzymes, allowing us to replace the last ones for several lab applications. We are reporting changes and modifications to standard protocols described. The standard protocols were modified, i.e. for the purification step of Taq, a temperature dependent procedure was designed. The enzymes produced were used in different applications, such as PCR, RT-PCR, PCR Multiplex and RAPDs molecular markers.
author	Manzur,María J. Muñoz,Rosana V. Lucero,Adrián A. Juri Ayub,Maximiliano Alvarez,Sergio E. Ciuffo,Gladys M.
author_facet	Manzur,María J. Muñoz,Rosana V. Lucero,Adrián A. Juri Ayub,Maximiliano Alvarez,Sergio E. Ciuffo,Gladys M.
author_sort	Manzur,María J.
title	Production of recombinant enzymes of wide use for research
title_short	Production of recombinant enzymes of wide use for research
title_full	Production of recombinant enzymes of wide use for research
title_fullStr	Production of recombinant enzymes of wide use for research
title_full_unstemmed	Production of recombinant enzymes of wide use for research
title_sort	production of recombinant enzymes of wide use for research
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300023
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_version_	1718441750208446464

Production of recombinant enzymes of wide use for research

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