RAPD and freezing resistance in Eucalyptus globulus

Eucalyptus globulus is the second most important forest species in Chile, after Pinus radiata. The main advantages of E. globulus are its fast growth (25 m³/ha/year) and its excellent wood quality for kraft pulp production. On the negative side, its low freezing tolerance has been an obstacle for th...

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Autores principales:	Fernández R.,Marta, Valenzuela A.,Sofía, Balocchi L.,Claudio
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	Eucalyptus globulus freezing resistance molecular markers RAPD
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300025
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spelling	oai:scielo:S0717-345820060003000252007-01-17RAPD and freezing resistance in Eucalyptus globulusFernández R.,MartaValenzuela A.,SofíaBalocchi L.,Claudio Eucalyptus globulus freezing resistance molecular markers RAPD Eucalyptus globulus is the second most important forest species in Chile, after Pinus radiata. The main advantages of E. globulus are its fast growth (25 m³/ha/year) and its excellent wood quality for kraft pulp production. On the negative side, its low freezing tolerance has been an obstacle for the expansion of plantations, specifically on the foothills of the Andes. The difference in the freezing resistance between clones of E. globulus has a genetic base and, therefore, it could be detected through DNA molecular markers. Fifteen clones of E. globulus, eight classified as freezing resistant and seven as freezing sensitive were analyzed using the technique of RAPD, in order to obtain molecular markers that could differentiate between freezing sensitive and resistant clones. Eighteen primers amplified reproducible bands. Three bands were only present in freezing resistant clones, two bands of 768 bp, 602 bp obtained with UBC 218 primer and one band of 248 bp obtained with UBC 237 primer. The preliminary results indicate that polymorphism can be observed with the primers employed, but it is not possible to associate the bands with the cold resistance or susceptibility in E. globulus.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.3 20062006-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300025en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Eucalyptus globulus freezing resistance molecular markers RAPD
spellingShingle	Eucalyptus globulus freezing resistance molecular markers RAPD Fernández R.,Marta Valenzuela A.,Sofía Balocchi L.,Claudio RAPD and freezing resistance in Eucalyptus globulus
description	Eucalyptus globulus is the second most important forest species in Chile, after Pinus radiata. The main advantages of E. globulus are its fast growth (25 m³/ha/year) and its excellent wood quality for kraft pulp production. On the negative side, its low freezing tolerance has been an obstacle for the expansion of plantations, specifically on the foothills of the Andes. The difference in the freezing resistance between clones of E. globulus has a genetic base and, therefore, it could be detected through DNA molecular markers. Fifteen clones of E. globulus, eight classified as freezing resistant and seven as freezing sensitive were analyzed using the technique of RAPD, in order to obtain molecular markers that could differentiate between freezing sensitive and resistant clones. Eighteen primers amplified reproducible bands. Three bands were only present in freezing resistant clones, two bands of 768 bp, 602 bp obtained with UBC 218 primer and one band of 248 bp obtained with UBC 237 primer. The preliminary results indicate that polymorphism can be observed with the primers employed, but it is not possible to associate the bands with the cold resistance or susceptibility in E. globulus.
author	Fernández R.,Marta Valenzuela A.,Sofía Balocchi L.,Claudio
author_facet	Fernández R.,Marta Valenzuela A.,Sofía Balocchi L.,Claudio
author_sort	Fernández R.,Marta
title	RAPD and freezing resistance in Eucalyptus globulus
title_short	RAPD and freezing resistance in Eucalyptus globulus
title_full	RAPD and freezing resistance in Eucalyptus globulus
title_fullStr	RAPD and freezing resistance in Eucalyptus globulus
title_full_unstemmed	RAPD and freezing resistance in Eucalyptus globulus
title_sort	rapd and freezing resistance in eucalyptus globulus
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300025
work_keys_str_mv	AT fernandezrmarta rapdandfreezingresistanceineucalyptusglobulus AT valenzuelaasofia rapdandfreezingresistanceineucalyptusglobulus AT balocchilclaudio rapdandfreezingresistanceineucalyptusglobulus
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RAPD and freezing resistance in Eucalyptus globulus

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