Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment

The phytopathogenic bacteria Agrobacterium rhizogenes genetically transforms plants by transferring a portion of the resident Ri plasmid, the T-DNA to the plant. Plant species differ in their temporal competence for transformation. But various physical and chemical methods are found to enhance trans...

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Autores principales: Kumar,Vinod, Sharma,Ashwani, Narasimha Prasad,Bellur Chayapathy, Bhaskar Gururaj,Harishchandra, Aswathanarayana Ravishankar,Gokare
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2006
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400002
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spelling oai:scielo:S0717-345820060004000022007-01-12Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatmentKumar,VinodSharma,AshwaniNarasimha Prasad,Bellur ChayapathyBhaskar Gururaj,HarishchandraAswathanarayana Ravishankar,Gokare Agrobacterium rhizogenes hairy roots Nicotiana tabacum transformation frequency ultra-sonication The phytopathogenic bacteria Agrobacterium rhizogenes genetically transforms plants by transferring a portion of the resident Ri plasmid, the T-DNA to the plant. Plant species differ in their temporal competence for transformation. But various physical and chemical methods are found to enhance transformation frequency. Agrobacterium rhizogenes mediated transformation efficiency was assessed under the influence of sonication, calcium treatment, acetosyringone and macerating enzymes in suitable combinations in Nicotiana tabacum as a model system. Manual wounding resulted in 21% transformation frequency. Where as sonication resulted in 2.2 fold increase, followed by sonication with CaCl2 treatment resulted in 2.5 fold increase and sonication with acetosyringone treatment resulted in 4.1 fold increase in transformation frequency. However, sonication with macerating enzyme treatment resulted in 1.5 to 5.25-fold decrease in transformation frequency. Micro wounding through sonication followed by acetosyringone treatment enhanced transformation frequency substantially. The results of this study may be very useful in genetic manipulation of plants by Agrobacterium rhizogenes mediated gene delivery to higher plants, which are recalcitrant to A. tumefaciens mediated genetic manipulation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.4 20062006-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400002en
institution Scielo Chile
collection Scielo Chile
language English
topic Agrobacterium rhizogenes
hairy roots
Nicotiana tabacum
transformation frequency
ultra-sonication
spellingShingle Agrobacterium rhizogenes
hairy roots
Nicotiana tabacum
transformation frequency
ultra-sonication
Kumar,Vinod
Sharma,Ashwani
Narasimha Prasad,Bellur Chayapathy
Bhaskar Gururaj,Harishchandra
Aswathanarayana Ravishankar,Gokare
Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment
description The phytopathogenic bacteria Agrobacterium rhizogenes genetically transforms plants by transferring a portion of the resident Ri plasmid, the T-DNA to the plant. Plant species differ in their temporal competence for transformation. But various physical and chemical methods are found to enhance transformation frequency. Agrobacterium rhizogenes mediated transformation efficiency was assessed under the influence of sonication, calcium treatment, acetosyringone and macerating enzymes in suitable combinations in Nicotiana tabacum as a model system. Manual wounding resulted in 21% transformation frequency. Where as sonication resulted in 2.2 fold increase, followed by sonication with CaCl2 treatment resulted in 2.5 fold increase and sonication with acetosyringone treatment resulted in 4.1 fold increase in transformation frequency. However, sonication with macerating enzyme treatment resulted in 1.5 to 5.25-fold decrease in transformation frequency. Micro wounding through sonication followed by acetosyringone treatment enhanced transformation frequency substantially. The results of this study may be very useful in genetic manipulation of plants by Agrobacterium rhizogenes mediated gene delivery to higher plants, which are recalcitrant to A. tumefaciens mediated genetic manipulation.
author Kumar,Vinod
Sharma,Ashwani
Narasimha Prasad,Bellur Chayapathy
Bhaskar Gururaj,Harishchandra
Aswathanarayana Ravishankar,Gokare
author_facet Kumar,Vinod
Sharma,Ashwani
Narasimha Prasad,Bellur Chayapathy
Bhaskar Gururaj,Harishchandra
Aswathanarayana Ravishankar,Gokare
author_sort Kumar,Vinod
title Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment
title_short Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment
title_full Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment
title_fullStr Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment
title_full_unstemmed Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment
title_sort agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2006
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400002
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AT sharmaashwani agrobacteriumrhizogenesmediatedgenetictransformationresultinginhairyrootformationisenhancedbyultrasonicationandacetosyringonetreatment
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