Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis

A myrosinase (thioglucoside glucohydrolase or thioglucosidase, EC 3.2.3.147) producing fungus, Aspergillus sp. NR4617, was newly isolated from decayed soil sample obtained in Thailand and was subjected to single exposure to two chemical mutagens, ethyl methanesulfonate (EMS) and N-methyl-N'-nit...

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Autores principales:	Rakariyatham,Nuansri, Butr-Indr,Bordin, Niamsup,Hataichanoke, Shank,Lalida
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	allylisothiocyanate Aspergillus sp. b-thioglucosidase myrosinase EMS mutagenesis MNNG mutagenesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400005
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spelling	oai:scielo:S0717-345820060004000052007-01-12Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesisRakariyatham,NuansriButr-Indr,BordinNiamsup,HataichanokeShank,Lalida allylisothiocyanate Aspergillus sp. b-thioglucosidase myrosinase EMS mutagenesis MNNG mutagenesis A myrosinase (thioglucoside glucohydrolase or thioglucosidase, EC 3.2.3.147) producing fungus, Aspergillus sp. NR4617, was newly isolated from decayed soil sample obtained in Thailand and was subjected to single exposure to two chemical mutagens, ethyl methanesulfonate (EMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Its myrosinase production was selected on low cost medium prepared from mustard seed cake (Brassica juncea). Studies of production and stability of the enzyme showed that EMS mutagenesis increased myrosinase activity. Aspergillus sp. NR4617E1 produced myrosinase 1.90 U ml-1 at 36 hrs of the cultivation equivalent to 171% of the enzyme production in wild-type. The stability studies revealed that myrosinase from the mutant strains retained activity similar to wild-type at 30ºC. Aspergillus sp. NR4617E1 degraded 10 mM of glucosinolate completely in 36 hrs. Enhanced myrosinase production and high yields of products (allylisothiocyanate) demonstrated that this mutant could be a new found candidate for feed detoxification and industrial allylisothiocyanate production.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.4 20062006-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400005en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	allylisothiocyanate Aspergillus sp. b-thioglucosidase myrosinase EMS mutagenesis MNNG mutagenesis
spellingShingle	allylisothiocyanate Aspergillus sp. b-thioglucosidase myrosinase EMS mutagenesis MNNG mutagenesis Rakariyatham,Nuansri Butr-Indr,Bordin Niamsup,Hataichanoke Shank,Lalida Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis
description	A myrosinase (thioglucoside glucohydrolase or thioglucosidase, EC 3.2.3.147) producing fungus, Aspergillus sp. NR4617, was newly isolated from decayed soil sample obtained in Thailand and was subjected to single exposure to two chemical mutagens, ethyl methanesulfonate (EMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Its myrosinase production was selected on low cost medium prepared from mustard seed cake (Brassica juncea). Studies of production and stability of the enzyme showed that EMS mutagenesis increased myrosinase activity. Aspergillus sp. NR4617E1 produced myrosinase 1.90 U ml-1 at 36 hrs of the cultivation equivalent to 171% of the enzyme production in wild-type. The stability studies revealed that myrosinase from the mutant strains retained activity similar to wild-type at 30ºC. Aspergillus sp. NR4617E1 degraded 10 mM of glucosinolate completely in 36 hrs. Enhanced myrosinase production and high yields of products (allylisothiocyanate) demonstrated that this mutant could be a new found candidate for feed detoxification and industrial allylisothiocyanate production.
author	Rakariyatham,Nuansri Butr-Indr,Bordin Niamsup,Hataichanoke Shank,Lalida
author_facet	Rakariyatham,Nuansri Butr-Indr,Bordin Niamsup,Hataichanoke Shank,Lalida
author_sort	Rakariyatham,Nuansri
title	Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis
title_short	Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis
title_full	Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis
title_fullStr	Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis
title_full_unstemmed	Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis
title_sort	improvement of myrosinase activity of aspergillus sp. nr4617 by chemical mutagenesis
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400005
work_keys_str_mv	AT rakariyathamnuansri improvementofmyrosinaseactivityofaspergillusspnr4617bychemicalmutagenesis AT butrindrbordin improvementofmyrosinaseactivityofaspergillusspnr4617bychemicalmutagenesis AT niamsuphataichanoke improvementofmyrosinaseactivityofaspergillusspnr4617bychemicalmutagenesis AT shanklalida improvementofmyrosinaseactivityofaspergillusspnr4617bychemicalmutagenesis
_version_	1718441753861685248

Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis

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