Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method

The combined effects of the processing parameters for exopolysaccharides production by Rhizobium sp. was studied using the experimental design and response surface methodology. The experiments were carried out using a fermenter with 20 L capacity, as the reactor. All processing parameters were onlin...

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Autores principales:	Pereira Duta,Flávia, Pessôa de França,Francisca, de Almeida Lopes,Léa Maria
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	exopolysaccharides experimental design response surface method Rhizobium sp.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400007
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spelling	oai:scielo:S0717-345820060004000072007-01-12Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface methodPereira Duta,FláviaPessôa de França,Franciscade Almeida Lopes,Léa Maria exopolysaccharides experimental design response surface method Rhizobium sp. The combined effects of the processing parameters for exopolysaccharides production by Rhizobium sp. was studied using the experimental design and response surface methodology. The experiments were carried out using a fermenter with 20 L capacity, as the reactor. All processing parameters were online monitored. The temperature [(30 ± 1)ºC] and pH value (7.0 ± 0.1) were kept constant throughout the experimental time. As statistical tools, a complete 2³ factorial planning with central point and response surface were used to study the interactions among three relevant variables of the fermentation process: calcium carbonate concentration, aeration and agitation. The processing parameters setup for reaching a maximum response for exopolysaccharides production was obtained when applying the highest values for calcium carbonate concentration (1.1 g/L), aeration (1.3 vvm) and agitation (800 rpm). In addition, the combination of these optimum processing parameters yielded Y P/S (g/g) = 0.35.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.4 20062006-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400007en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	exopolysaccharides experimental design response surface method Rhizobium sp.
spellingShingle	exopolysaccharides experimental design response surface method Rhizobium sp. Pereira Duta,Flávia Pessôa de França,Francisca de Almeida Lopes,Léa Maria Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method
description	The combined effects of the processing parameters for exopolysaccharides production by Rhizobium sp. was studied using the experimental design and response surface methodology. The experiments were carried out using a fermenter with 20 L capacity, as the reactor. All processing parameters were online monitored. The temperature [(30 ± 1)ºC] and pH value (7.0 ± 0.1) were kept constant throughout the experimental time. As statistical tools, a complete 2³ factorial planning with central point and response surface were used to study the interactions among three relevant variables of the fermentation process: calcium carbonate concentration, aeration and agitation. The processing parameters setup for reaching a maximum response for exopolysaccharides production was obtained when applying the highest values for calcium carbonate concentration (1.1 g/L), aeration (1.3 vvm) and agitation (800 rpm). In addition, the combination of these optimum processing parameters yielded Y P/S (g/g) = 0.35.
author	Pereira Duta,Flávia Pessôa de França,Francisca de Almeida Lopes,Léa Maria
author_facet	Pereira Duta,Flávia Pessôa de França,Francisca de Almeida Lopes,Léa Maria
author_sort	Pereira Duta,Flávia
title	Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method
title_short	Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method
title_full	Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method
title_fullStr	Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method
title_full_unstemmed	Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method
title_sort	optimization of culture conditions for exopolysaccharides production in rhizobium sp. using the response surface method
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400007
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Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method

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