The lift pool method for isolation of cDNA clones from lambda phage libraries

The lift pool method for isolation of cDNA clones from lambda phage libraries

A PCR based strategy was developed to screen a Xenopus oocyte λgt10 cDNA library. The PCR-based lift pool (LP) method follows the same two tiered strategy as conventional screening of phage libraries by filter hybridization. Two rounds of plating, one at high density to detect the clone,...

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Autores principales: LeBlanc-Straceski,Janine, Sobrado,Pablo, Betz,Sharon, Zerfas,Julie, Morgan,Karen
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2006
Materias:
cDNA
lambda
non-radioactive
PCR
plaque
screening
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400012
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