Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia sp. cells

Isomaltulose is a structural isomer of sucrose commercially used in food industries. Glucosyltransferase produced by Erwinia sp. D12 catalyses an intramolecular transglucosylation of sucrose giving isomaltulose. The Experimental design and response surface methodology were applied for the optimizati...

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Autores principales:	Yukio Kawaguti,Haroldo, Manrich,Eiric, Harumi Sato,Hélia
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	batch process Erwinia sp. free cells glucosyltransferase isomaltulose response surface methodology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500003
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spelling	oai:scielo:S0717-345820060005000032007-01-04Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia sp. cellsYukio Kawaguti,HaroldoManrich,EiricHarumi Sato,Hélia batch process Erwinia sp. free cells glucosyltransferase isomaltulose response surface methodology Isomaltulose is a structural isomer of sucrose commercially used in food industries. Glucosyltransferase produced by Erwinia sp. D12 catalyses an intramolecular transglucosylation of sucrose giving isomaltulose. The Experimental design and response surface methodology were applied for the optimization of the nutrient concentration in the culture medium for the enzyme production in shaken flasks at 200 rpm and 30ºC. The three variables involved in this study were sugar cane molasses, bacteriological peptone and yeast extract Prodex Lac SD®. The statistical analysis of the results showed that, in the range studied, all the factors had a significant effect (p < 0.05) on glucosyltransferase production and the highest enzyme activity was observed in culture medium containing sugar cane molasses (160 g/L), bacteriological peptone (20 g/L) and yeast extract Prodex Lac SD® (15 g/L). Maximum glucosyltransferase activity of 29.88 U/mL was achieved in a 6.6-L fermenter using the optimized medium. Free Erwinia sp. D12 cells were used for isomaltulose production from sucrose during fifteen successive batches. The final isomaltulose concentration of 75.6% obtained in the first batch increased to 77.21% (mean value) in the other fourteen batches and the productivity of 1.1 g/L x hr was obtained in batch process.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.5 20062006-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500003en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	batch process Erwinia sp. free cells glucosyltransferase isomaltulose response surface methodology
spellingShingle	batch process Erwinia sp. free cells glucosyltransferase isomaltulose response surface methodology Yukio Kawaguti,Haroldo Manrich,Eiric Harumi Sato,Hélia Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia sp. cells
description	Isomaltulose is a structural isomer of sucrose commercially used in food industries. Glucosyltransferase produced by Erwinia sp. D12 catalyses an intramolecular transglucosylation of sucrose giving isomaltulose. The Experimental design and response surface methodology were applied for the optimization of the nutrient concentration in the culture medium for the enzyme production in shaken flasks at 200 rpm and 30ºC. The three variables involved in this study were sugar cane molasses, bacteriological peptone and yeast extract Prodex Lac SD®. The statistical analysis of the results showed that, in the range studied, all the factors had a significant effect (p < 0.05) on glucosyltransferase production and the highest enzyme activity was observed in culture medium containing sugar cane molasses (160 g/L), bacteriological peptone (20 g/L) and yeast extract Prodex Lac SD® (15 g/L). Maximum glucosyltransferase activity of 29.88 U/mL was achieved in a 6.6-L fermenter using the optimized medium. Free Erwinia sp. D12 cells were used for isomaltulose production from sucrose during fifteen successive batches. The final isomaltulose concentration of 75.6% obtained in the first batch increased to 77.21% (mean value) in the other fourteen batches and the productivity of 1.1 g/L x hr was obtained in batch process.
author	Yukio Kawaguti,Haroldo Manrich,Eiric Harumi Sato,Hélia
author_facet	Yukio Kawaguti,Haroldo Manrich,Eiric Harumi Sato,Hélia
author_sort	Yukio Kawaguti,Haroldo
title	Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia sp. cells
title_short	Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia sp. cells
title_full	Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia sp. cells
title_fullStr	Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia sp. cells
title_full_unstemmed	Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia sp. cells
title_sort	application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free erwinia sp. cells
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500003
work_keys_str_mv	AT yukiokawagutiharoldo applicationofresponsesurfacemethodologyforglucosyltransferaseproductionandconversionofsucroseintoisomaltuloseusingfreeerwiniaspcells AT manricheiric applicationofresponsesurfacemethodologyforglucosyltransferaseproductionandconversionofsucroseintoisomaltuloseusingfreeerwiniaspcells AT harumisatohelia applicationofresponsesurfacemethodologyforglucosyltransferaseproductionandconversionofsucroseintoisomaltuloseusingfreeerwiniaspcells
_version_	1718441756707520512

Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia sp. cells

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