Continuous gluconic acid production by Aureobasidium pullulans with and without biomass retention

New alternative processes for the continuous production of gluconic acid by Aureobasidium pullulans, using biomass retention by cell immobilization or cross over filtration, are described in the present work. 315 g/l gluconic acid was continuously produced in chemostat cultures at 21 hrs residence t...

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Autores principales:	Anastassiadis,Savas, Rehm,Hans-Jürgen
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	biomass immobilization continuous fermentation cross over filtration gluconic acid fermentation reaction technique residence time
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500004
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spelling	oai:scielo:S0717-345820060005000042007-01-04Continuous gluconic acid production by Aureobasidium pullulans with and without biomass retentionAnastassiadis,SavasRehm,Hans-Jürgen biomass immobilization continuous fermentation cross over filtration gluconic acid fermentation reaction technique residence time New alternative processes for the continuous production of gluconic acid by Aureobasidium pullulans, using biomass retention by cell immobilization or cross over filtration, are described in the present work. 315 g/l gluconic acid was continuously produced in chemostat cultures at 21 hrs residence time without any biomass retention. 260 g/l gluconic acid was produced in fluidized bed reactor at 21 hrs residence time. The support carrier was overgrown resulting in limitations of oxygen transfer towards the inner layers of immobilized biomass. 375 g/l gluconic acid was produced under continuous cultivation at 22 hrs of residence time with a formation rate for the generic product of 17 g/(l x h) and a specific gluconic acid productivity of only 0.74 g/(g x h), using biomass retention by cross over filtration. 370 g/l were obtained at 19 hrs RT and 100% conversion with 25 g/l biomass and a formation rate of 19 g/(l x h). At 100% conversion, a selectivity of only 78% was determined at 22 hrs and of 77% at 19 hrs RT, because of the very high biomass concentration. Biomass retention makes it possible to break the existing link between growth and residence time.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.5 20062006-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500004en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	biomass immobilization continuous fermentation cross over filtration gluconic acid fermentation reaction technique residence time
spellingShingle	biomass immobilization continuous fermentation cross over filtration gluconic acid fermentation reaction technique residence time Anastassiadis,Savas Rehm,Hans-Jürgen Continuous gluconic acid production by Aureobasidium pullulans with and without biomass retention
description	New alternative processes for the continuous production of gluconic acid by Aureobasidium pullulans, using biomass retention by cell immobilization or cross over filtration, are described in the present work. 315 g/l gluconic acid was continuously produced in chemostat cultures at 21 hrs residence time without any biomass retention. 260 g/l gluconic acid was produced in fluidized bed reactor at 21 hrs residence time. The support carrier was overgrown resulting in limitations of oxygen transfer towards the inner layers of immobilized biomass. 375 g/l gluconic acid was produced under continuous cultivation at 22 hrs of residence time with a formation rate for the generic product of 17 g/(l x h) and a specific gluconic acid productivity of only 0.74 g/(g x h), using biomass retention by cross over filtration. 370 g/l were obtained at 19 hrs RT and 100% conversion with 25 g/l biomass and a formation rate of 19 g/(l x h). At 100% conversion, a selectivity of only 78% was determined at 22 hrs and of 77% at 19 hrs RT, because of the very high biomass concentration. Biomass retention makes it possible to break the existing link between growth and residence time.
author	Anastassiadis,Savas Rehm,Hans-Jürgen
author_facet	Anastassiadis,Savas Rehm,Hans-Jürgen
author_sort	Anastassiadis,Savas
title	Continuous gluconic acid production by Aureobasidium pullulans with and without biomass retention
title_short	Continuous gluconic acid production by Aureobasidium pullulans with and without biomass retention
title_full	Continuous gluconic acid production by Aureobasidium pullulans with and without biomass retention
title_fullStr	Continuous gluconic acid production by Aureobasidium pullulans with and without biomass retention
title_full_unstemmed	Continuous gluconic acid production by Aureobasidium pullulans with and without biomass retention
title_sort	continuous gluconic acid production by aureobasidium pullulans with and without biomass retention
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500004
work_keys_str_mv	AT anastassiadissavas continuousgluconicacidproductionbyaureobasidiumpullulanswithandwithoutbiomassretention AT rehmhansjurgen continuousgluconicacidproductionbyaureobasidiumpullulanswithandwithoutbiomassretention
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Continuous gluconic acid production by Aureobasidium pullulans with and without biomass retention

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