Designing and validation of genus-specific primers for human gut flora study

The aim of this study, was to design and validate 16S rRNA targeted oligonucleotide genus specific primers for amplifying the predominant members of gut flora using polymerase chain reaction. Primers were validated against human faecal samples. Gut flora of a normal individual was compared with that...

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Autores principales:	Rekha,Rani, Alam Rizvi,Moshahid, Jaishree,Paul
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	16S rRNA based primers anaerobic bacteria diarrhoea patient polymerase chain reaction
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500005
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spelling	oai:scielo:S0717-345820060005000052007-01-04Designing and validation of genus-specific primers for human gut flora studyRekha,RaniAlam Rizvi,MoshahidJaishree,Paul 16S rRNA based primers anaerobic bacteria diarrhoea patient polymerase chain reaction The aim of this study, was to design and validate 16S rRNA targeted oligonucleotide genus specific primers for amplifying the predominant members of gut flora using polymerase chain reaction. Primers were validated against human faecal samples. Gut flora of a normal individual was compared with that of two diseased individuals. Our observations showed that the genera Lactobacillus, Bacteroides, Peptococcus, Bifidobacterium, and E. coli were invariably present in all studied subjects however, the absence of butyrate producing bacteria Ruminococcus and Peptostreptococcus were significant. Presence of the members of the genus, Campylobacter in both the diseased samples were also unusual.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.5 20062006-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500005en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	16S rRNA based primers anaerobic bacteria diarrhoea patient polymerase chain reaction
spellingShingle	16S rRNA based primers anaerobic bacteria diarrhoea patient polymerase chain reaction Rekha,Rani Alam Rizvi,Moshahid Jaishree,Paul Designing and validation of genus-specific primers for human gut flora study
description	The aim of this study, was to design and validate 16S rRNA targeted oligonucleotide genus specific primers for amplifying the predominant members of gut flora using polymerase chain reaction. Primers were validated against human faecal samples. Gut flora of a normal individual was compared with that of two diseased individuals. Our observations showed that the genera Lactobacillus, Bacteroides, Peptococcus, Bifidobacterium, and E. coli were invariably present in all studied subjects however, the absence of butyrate producing bacteria Ruminococcus and Peptostreptococcus were significant. Presence of the members of the genus, Campylobacter in both the diseased samples were also unusual.
author	Rekha,Rani Alam Rizvi,Moshahid Jaishree,Paul
author_facet	Rekha,Rani Alam Rizvi,Moshahid Jaishree,Paul
author_sort	Rekha,Rani
title	Designing and validation of genus-specific primers for human gut flora study
title_short	Designing and validation of genus-specific primers for human gut flora study
title_full	Designing and validation of genus-specific primers for human gut flora study
title_fullStr	Designing and validation of genus-specific primers for human gut flora study
title_full_unstemmed	Designing and validation of genus-specific primers for human gut flora study
title_sort	designing and validation of genus-specific primers for human gut flora study
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500005
work_keys_str_mv	AT rekharani designingandvalidationofgenusspecificprimersforhumangutflorastudy AT alamrizvimoshahid designingandvalidationofgenusspecificprimersforhumangutflorastudy AT jaishreepaul designingandvalidationofgenusspecificprimersforhumangutflorastudy
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Designing and validation of genus-specific primers for human gut flora study

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