Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L.
Genetically transformed roots of red beet produce copious levels of peroxidase (POD) - a multifunctional enzyme with a number of commercial applications. In an effort to elicit the POD activity, the cultures were treated with biotic elicitors such as dry cell powders of microbial cultures (0.1-0.5%...
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Pontificia Universidad Católica de Valparaíso
2006
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oai:scielo:S0717-345820060005000062007-01-04Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L.Rudrappa,ThimmarajuNeelwarne,BhagyalakshmiLakshmanan,VenkatachalamReddampalli Venkataramareddy,SreedharGokare Aswathanarayana,Ravishankar Aspergillus calcium culture filtrate dried cell powder elicitor glutathione methyljasmonate Rhizophus thidiazuron Genetically transformed roots of red beet produce copious levels of peroxidase (POD) - a multifunctional enzyme with a number of commercial applications. In an effort to elicit the POD activity, the cultures were treated with biotic elicitors such as dry cell powders of microbial cultures (0.1-0.5% w/v) and the respective culture filtrates (1-5% v/v). Similarly, abiotic elicitors, particularly metal ions (2-8 folds of that present in the nutrient medium), the plant hormone Thidiazuron (at 0.25-1 ppm) and other bio-molecules such as Glutathione (at 0.5-10 mM) and Methyl jasmonate (at 20-100 µM) were used. It was observed that dry cell powder of Candida versatilis significantly elicited the enzyme activity (3.52-fold higher than the control) followed by glutathione (3.44-fold) and Rhizophus oligosporus (3.09-fold). Among abiotic elicitors, thidiazuron, Mg and Ca salts elicited 2.49, 3.03 and 2.8 fold activities respectively. While most of the biotic elicitors were effective when added on 15th day of culture, the abiotic elicitors were effective when added on 20th day. Combination of highly effective elicitors indicated that glutathione (1 mM) and dry cell powder of R. oligosporus caused a 4-fold enhancement in enzyme activity, accounting for 10.9 x 10(6) U L-1. The present study is the first report on red beet hairy roots where a large number of elicitors have been systematically screened and their probable involvements in eliciting POD activities have been discussed.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.5 20062006-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500006en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Aspergillus calcium culture filtrate dried cell powder elicitor glutathione methyljasmonate Rhizophus thidiazuron |
| spellingShingle |
Aspergillus calcium culture filtrate dried cell powder elicitor glutathione methyljasmonate Rhizophus thidiazuron Rudrappa,Thimmaraju Neelwarne,Bhagyalakshmi Lakshmanan,Venkatachalam Reddampalli Venkataramareddy,Sreedhar Gokare Aswathanarayana,Ravishankar Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L. |
| description |
Genetically transformed roots of red beet produce copious levels of peroxidase (POD) - a multifunctional enzyme with a number of commercial applications. In an effort to elicit the POD activity, the cultures were treated with biotic elicitors such as dry cell powders of microbial cultures (0.1-0.5% w/v) and the respective culture filtrates (1-5% v/v). Similarly, abiotic elicitors, particularly metal ions (2-8 folds of that present in the nutrient medium), the plant hormone Thidiazuron (at 0.25-1 ppm) and other bio-molecules such as Glutathione (at 0.5-10 mM) and Methyl jasmonate (at 20-100 µM) were used. It was observed that dry cell powder of Candida versatilis significantly elicited the enzyme activity (3.52-fold higher than the control) followed by glutathione (3.44-fold) and Rhizophus oligosporus (3.09-fold). Among abiotic elicitors, thidiazuron, Mg and Ca salts elicited 2.49, 3.03 and 2.8 fold activities respectively. While most of the biotic elicitors were effective when added on 15th day of culture, the abiotic elicitors were effective when added on 20th day. Combination of highly effective elicitors indicated that glutathione (1 mM) and dry cell powder of R. oligosporus caused a 4-fold enhancement in enzyme activity, accounting for 10.9 x 10(6) U L-1. The present study is the first report on red beet hairy roots where a large number of elicitors have been systematically screened and their probable involvements in eliciting POD activities have been discussed. |
| author |
Rudrappa,Thimmaraju Neelwarne,Bhagyalakshmi Lakshmanan,Venkatachalam Reddampalli Venkataramareddy,Sreedhar Gokare Aswathanarayana,Ravishankar |
| author_facet |
Rudrappa,Thimmaraju Neelwarne,Bhagyalakshmi Lakshmanan,Venkatachalam Reddampalli Venkataramareddy,Sreedhar Gokare Aswathanarayana,Ravishankar |
| author_sort |
Rudrappa,Thimmaraju |
| title |
Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L. |
| title_short |
Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L. |
| title_full |
Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L. |
| title_fullStr |
Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L. |
| title_full_unstemmed |
Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L. |
| title_sort |
elicitation of peroxidase activity in genetically transformed root cultures of beta vulgaris l. |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2006 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500006 |
| work_keys_str_mv |
AT rudrappathimmaraju elicitationofperoxidaseactivityingeneticallytransformedrootculturesofbetavulgarisl AT neelwarnebhagyalakshmi elicitationofperoxidaseactivityingeneticallytransformedrootculturesofbetavulgarisl AT lakshmananvenkatachalam elicitationofperoxidaseactivityingeneticallytransformedrootculturesofbetavulgarisl AT reddampallivenkataramareddysreedhar elicitationofperoxidaseactivityingeneticallytransformedrootculturesofbetavulgarisl AT gokareaswathanarayanaravishankar elicitationofperoxidaseactivityingeneticallytransformedrootculturesofbetavulgarisl |
| _version_ |
1718441757485563904 |