Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture

Insect cell culture has become increasingly useful for the production of heterologous proteins as well as of baculovirus polyhedra, and several different culture media formulations can be employed for this purpose. The goal of this work was to assess the potential of lyophylized milk whey ultra filt...

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Autores principales:	Batista,Fabiana R. X., Pereira,Carlos A., Mendonça,Ronaldo Z., Moraes,Angela M.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	baculovirus concentrated milk whey Grace's medium insect cells media formulation Spodoptera frugiperda Sf9
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500007
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spelling	oai:scielo:S0717-345820060005000072007-01-04Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in cultureBatista,Fabiana R. X.Pereira,Carlos A.Mendonça,Ronaldo Z.Moraes,Angela M. baculovirus concentrated milk whey Grace's medium insect cells media formulation Spodoptera frugiperda Sf9 Insect cell culture has become increasingly useful for the production of heterologous proteins as well as of baculovirus polyhedra, and several different culture media formulations can be employed for this purpose. The goal of this work was to assess the potential of lyophylized milk whey ultra filtration retentate (MWR) when associated to yeast extract (YE), glucose and Pluronic F68 (PF68) to partially replace fetal calf serum (FBS) in Spodoptera frugiperda Sf9 cells culture in Grace's medium, aiming AgMNPV baculovirus production. Batch cultivation results showed that the yeast extract and the milk whey concentrate effectively increased cell concentration to about half the level commonly verified for the Sf900II serum-free medium. A 4-fold increase in viable cell concentration was achieved when employing 5% (w/v) MWR, 8 g/L YE, 1% (v/v) of FBS and 2.7 g.l-1 of glucose, resulting in 1.6 x 10(7) polyhedra.ml-1 after infection with baculovirus.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.5 20062006-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500007en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	baculovirus concentrated milk whey Grace's medium insect cells media formulation Spodoptera frugiperda Sf9
spellingShingle	baculovirus concentrated milk whey Grace's medium insect cells media formulation Spodoptera frugiperda Sf9 Batista,Fabiana R. X. Pereira,Carlos A. Mendonça,Ronaldo Z. Moraes,Angela M. Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture
description	Insect cell culture has become increasingly useful for the production of heterologous proteins as well as of baculovirus polyhedra, and several different culture media formulations can be employed for this purpose. The goal of this work was to assess the potential of lyophylized milk whey ultra filtration retentate (MWR) when associated to yeast extract (YE), glucose and Pluronic F68 (PF68) to partially replace fetal calf serum (FBS) in Spodoptera frugiperda Sf9 cells culture in Grace's medium, aiming AgMNPV baculovirus production. Batch cultivation results showed that the yeast extract and the milk whey concentrate effectively increased cell concentration to about half the level commonly verified for the Sf900II serum-free medium. A 4-fold increase in viable cell concentration was achieved when employing 5% (w/v) MWR, 8 g/L YE, 1% (v/v) of FBS and 2.7 g.l-1 of glucose, resulting in 1.6 x 10(7) polyhedra.ml-1 after infection with baculovirus.
author	Batista,Fabiana R. X. Pereira,Carlos A. Mendonça,Ronaldo Z. Moraes,Angela M.
author_facet	Batista,Fabiana R. X. Pereira,Carlos A. Mendonça,Ronaldo Z. Moraes,Angela M.
author_sort	Batista,Fabiana R. X.
title	Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture
title_short	Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture
title_full	Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture
title_fullStr	Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture
title_full_unstemmed	Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture
title_sort	evaluation of concentrated milk whey as a supplement for sf9 spodoptera frugiperda cells in culture
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500007
work_keys_str_mv	AT batistafabianarx evaluationofconcentratedmilkwheyasasupplementforsf9spodopterafrugiperdacellsinculture AT pereiracarlosa evaluationofconcentratedmilkwheyasasupplementforsf9spodopterafrugiperdacellsinculture AT mendoncaronaldoz evaluationofconcentratedmilkwheyasasupplementforsf9spodopterafrugiperdacellsinculture AT moraesangelam evaluationofconcentratedmilkwheyasasupplementforsf9spodopterafrugiperdacellsinculture
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Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture

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