Application of statistical experimental methods to optimize medium for exopolymer production by newly isolated Halobacterium sp. SM5

The study investigated on medium optimization for production of exopolymer by a newly isolated bacterium, Halobacterium sp. SM5, using the mixture design and response surface method. The mixture experiment was designed by setting five parameters at the lowest and highest of concentrations: pseudo co...

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Autores principales:	Lungmann,Patcharee, Choorit,Wanna, Prasertsan,Poonsuk
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	exopolymer halophilic bacteria medium optimization mixture design response surface method
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100001
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spelling	oai:scielo:S0717-345820070001000012007-08-14Application of statistical experimental methods to optimize medium for exopolymer production by newly isolated Halobacterium sp. SM5Lungmann,PatchareeChoorit,WannaPrasertsan,Poonsuk exopolymer halophilic bacteria medium optimization mixture design response surface method The study investigated on medium optimization for production of exopolymer by a newly isolated bacterium, Halobacterium sp. SM5, using the mixture design and response surface method. The mixture experiment was designed by setting five parameters at the lowest and highest of concentrations: pseudo components design containing 0.15-0.25 glucose, 0.15-0.25 yeast extract, 0.35-0.45 MgSO4·7H2O, 0.1-0.2 vitamin casamino acid and 0.02-0.06 KCl. The results of the mixture design revealed that the effect of nutrients or elements on the exopolymer produced by the strain SM5 were in the order of MgSO4·7H2O > yeast extract > vitamin casamino acid > KCl > glucose, respectively. The suitable medium recipe for enhancement the exopolymer production was 7.43 g/l glucose, 12.38 g/l yeast extract, 17.33 g/l MgSO4·7H2O, 9.9 g/l vitamin casamino acid and 2.48 g/l KCl. The exopolymer to be produced by the strain SM5 was 2.25 g/l, which was higher than that obtained in the original medium (1.3 times). The yield of exopolymer was 2.13 g/l to be obtained in medium containing 7.43 g/l glucose, 11.37 g/l yeast extract, 22.28 g/l MgSO4·7H2O, 7.44 g/l vitamin casamino acid and 0.99 g/l KCl which was predicted by response surface methodology. However, under an experiment, the yield of exopolymer was 2.08 ± 0.0020 g/linfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.1 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100001en10.4067/S0717-34582007000100001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	exopolymer halophilic bacteria medium optimization mixture design response surface method
spellingShingle	exopolymer halophilic bacteria medium optimization mixture design response surface method Lungmann,Patcharee Choorit,Wanna Prasertsan,Poonsuk Application of statistical experimental methods to optimize medium for exopolymer production by newly isolated Halobacterium sp. SM5
description	The study investigated on medium optimization for production of exopolymer by a newly isolated bacterium, Halobacterium sp. SM5, using the mixture design and response surface method. The mixture experiment was designed by setting five parameters at the lowest and highest of concentrations: pseudo components design containing 0.15-0.25 glucose, 0.15-0.25 yeast extract, 0.35-0.45 MgSO4·7H2O, 0.1-0.2 vitamin casamino acid and 0.02-0.06 KCl. The results of the mixture design revealed that the effect of nutrients or elements on the exopolymer produced by the strain SM5 were in the order of MgSO4·7H2O > yeast extract > vitamin casamino acid > KCl > glucose, respectively. The suitable medium recipe for enhancement the exopolymer production was 7.43 g/l glucose, 12.38 g/l yeast extract, 17.33 g/l MgSO4·7H2O, 9.9 g/l vitamin casamino acid and 2.48 g/l KCl. The exopolymer to be produced by the strain SM5 was 2.25 g/l, which was higher than that obtained in the original medium (1.3 times). The yield of exopolymer was 2.13 g/l to be obtained in medium containing 7.43 g/l glucose, 11.37 g/l yeast extract, 22.28 g/l MgSO4·7H2O, 7.44 g/l vitamin casamino acid and 0.99 g/l KCl which was predicted by response surface methodology. However, under an experiment, the yield of exopolymer was 2.08 ± 0.0020 g/l
author	Lungmann,Patcharee Choorit,Wanna Prasertsan,Poonsuk
author_facet	Lungmann,Patcharee Choorit,Wanna Prasertsan,Poonsuk
author_sort	Lungmann,Patcharee
title	Application of statistical experimental methods to optimize medium for exopolymer production by newly isolated Halobacterium sp. SM5
title_short	Application of statistical experimental methods to optimize medium for exopolymer production by newly isolated Halobacterium sp. SM5
title_full	Application of statistical experimental methods to optimize medium for exopolymer production by newly isolated Halobacterium sp. SM5
title_fullStr	Application of statistical experimental methods to optimize medium for exopolymer production by newly isolated Halobacterium sp. SM5
title_full_unstemmed	Application of statistical experimental methods to optimize medium for exopolymer production by newly isolated Halobacterium sp. SM5
title_sort	application of statistical experimental methods to optimize medium for exopolymer production by newly isolated halobacterium sp. sm5
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100001
work_keys_str_mv	AT lungmannpatcharee applicationofstatisticalexperimentalmethodstooptimizemediumforexopolymerproductionbynewlyisolatedhalobacteriumspsm5 AT chooritwanna applicationofstatisticalexperimentalmethodstooptimizemediumforexopolymerproductionbynewlyisolatedhalobacteriumspsm5 AT prasertsanpoonsuk applicationofstatisticalexperimentalmethodstooptimizemediumforexopolymerproductionbynewlyisolatedhalobacteriumspsm5
_version_	1718441760518045696

Application of statistical experimental methods to optimize medium for exopolymer production by newly isolated Halobacterium sp. SM5

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