Growth of Cunninghamella elegans UCP 542 and production of chitin and chitosan using yam bean medium

Microbiological processes were used for chitin and chitosan productions by Cunninghamella elegans (UCP 542) grown in a new economic culture medium. The assay was carried out to evaluate the growth of C. elegans using yam bean (Pachyrhizus erosus L. Urban) medium, in different times of growth (24, 48...

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Autores principales:	Stamford,Thayza Christina Montenegro, Stamford,Tânia Lucia Montenegro, Stamford,Newton Pereira, Barros Neto,Benicio de, Campos-Takaki,Galba Maria de
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	biopolymers chitin chitosan
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100006
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spelling	oai:scielo:S0717-345820070001000062007-08-14Growth of Cunninghamella elegans UCP 542 and production of chitin and chitosan using yam bean mediumStamford,Thayza Christina MontenegroStamford,Tânia Lucia MontenegroStamford,Newton PereiraBarros Neto,Benicio deCampos-Takaki,Galba Maria de biopolymers chitin chitosan Microbiological processes were used for chitin and chitosan productions by Cunninghamella elegans (UCP 542) grown in a new economic culture medium. The assay was carried out to evaluate the growth of C. elegans using yam bean (Pachyrhizus erosus L. Urban) medium, in different times of growth (24, 48, 72 and 96 hrs), incubated at 28ºC in an orbital shaker at 150 rpm. The lyophilized biomass was determined by gravimetry. The polysaccharides were extracted by alkali-acid treatment, and characterized by infrared spectroscopy, titration and viscosity. C. elegans grown in the yam bean medium and produced higher yields of biomass (24.3 g/ mL) in 96 hrs. The high level was chitosan (66 mg/g), and chitin (440 mg/g) were produced at 48 and 72 hrs of growth, respectively. The polysaccharides showed degree of deacetilation and viscosimetric molecular weight as: 6.2% and 3.25 x10(4) g/mol for chitin, and 85% and 2.72 x 10(4) g/mol for chitosan, respectively. The results obtained suggest high biotechnological potential of yam bean as an economic source to produce chitin and chitosan by C. elegans. In addition, the new medium using yam bean for production of the chitin and chitosan may be used for many purposes to reduce the cost price of fermentation processes.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.1 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100006en10.4067/S0717-34582007000100006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	biopolymers chitin chitosan
spellingShingle	biopolymers chitin chitosan Stamford,Thayza Christina Montenegro Stamford,Tânia Lucia Montenegro Stamford,Newton Pereira Barros Neto,Benicio de Campos-Takaki,Galba Maria de Growth of Cunninghamella elegans UCP 542 and production of chitin and chitosan using yam bean medium
description	Microbiological processes were used for chitin and chitosan productions by Cunninghamella elegans (UCP 542) grown in a new economic culture medium. The assay was carried out to evaluate the growth of C. elegans using yam bean (Pachyrhizus erosus L. Urban) medium, in different times of growth (24, 48, 72 and 96 hrs), incubated at 28ºC in an orbital shaker at 150 rpm. The lyophilized biomass was determined by gravimetry. The polysaccharides were extracted by alkali-acid treatment, and characterized by infrared spectroscopy, titration and viscosity. C. elegans grown in the yam bean medium and produced higher yields of biomass (24.3 g/ mL) in 96 hrs. The high level was chitosan (66 mg/g), and chitin (440 mg/g) were produced at 48 and 72 hrs of growth, respectively. The polysaccharides showed degree of deacetilation and viscosimetric molecular weight as: 6.2% and 3.25 x10(4) g/mol for chitin, and 85% and 2.72 x 10(4) g/mol for chitosan, respectively. The results obtained suggest high biotechnological potential of yam bean as an economic source to produce chitin and chitosan by C. elegans. In addition, the new medium using yam bean for production of the chitin and chitosan may be used for many purposes to reduce the cost price of fermentation processes.
author	Stamford,Thayza Christina Montenegro Stamford,Tânia Lucia Montenegro Stamford,Newton Pereira Barros Neto,Benicio de Campos-Takaki,Galba Maria de
author_facet	Stamford,Thayza Christina Montenegro Stamford,Tânia Lucia Montenegro Stamford,Newton Pereira Barros Neto,Benicio de Campos-Takaki,Galba Maria de
author_sort	Stamford,Thayza Christina Montenegro
title	Growth of Cunninghamella elegans UCP 542 and production of chitin and chitosan using yam bean medium
title_short	Growth of Cunninghamella elegans UCP 542 and production of chitin and chitosan using yam bean medium
title_full	Growth of Cunninghamella elegans UCP 542 and production of chitin and chitosan using yam bean medium
title_fullStr	Growth of Cunninghamella elegans UCP 542 and production of chitin and chitosan using yam bean medium
title_full_unstemmed	Growth of Cunninghamella elegans UCP 542 and production of chitin and chitosan using yam bean medium
title_sort	growth of cunninghamella elegans ucp 542 and production of chitin and chitosan using yam bean medium
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100006
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Growth of Cunninghamella elegans UCP 542 and production of chitin and chitosan using yam bean medium

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