Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies

Samples of green colonial unicellular microalga Botryococcus braunii were collected from Bear Shola Falls at Kodaikanal (latitude 10.31 N and longitude 77.32 E), Tamil Nadu, India. Specimens were isolated, cultured and examined for its hydrocarbon content, morphological features and DNA structural r...

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Autores principales:	Dayananda,Chandrappa, Sarada,Ravi, Kumar,Vinod, Ravishankar,Gokare Aswathanarayana
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	biomass carbon dioxide carotenoids fatty acids inter simple sequence repeats saturated hydrocarbons
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100008
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spelling	oai:scielo:S0717-345820070001000082007-08-14Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodiesDayananda,ChandrappaSarada,RaviKumar,VinodRavishankar,Gokare Aswathanarayana biomass carbon dioxide carotenoids fatty acids inter simple sequence repeats saturated hydrocarbons Samples of green colonial unicellular microalga Botryococcus braunii were collected from Bear Shola Falls at Kodaikanal (latitude 10.31 N and longitude 77.32 E), Tamil Nadu, India. Specimens were isolated, cultured and examined for its hydrocarbon content, morphological features and DNA structural resemblance with the known strain to confirm its identity. Inter simple sequence repeats (ISSR) finger printing revealed strong genetic similarity among the authentic strain (B. braunii N-836) and the Indian isolated strain (B. braunii CFTRI- Bb1) from Kodaikanal. The type of hydrocarbons produced by the Kodaikanal isolates were analyzed and identified as saturated hydrocarbons in the range of C21 to C33 by GCMS. Tetracosane and octacosane were found as the major components among the saturated hydrocarbons produced by this alga, constituting 17.6% and 14.8% respectively. Hydrocarbon content of the organism was in the range of 13-18% of its dry biomass. The fat content of the organism was found to be 22% (w/w). Palmitic and oleic acids were found to be major fatty acids produced by the alga. Lutein and β-carotene were found to be the major carotenoids and constituted about 64.1% and 25.1% respectively of the total carotenoids. Based on ISSR finger printing and hydrocarbon analyses the new algal strain from Kodaikanal was identified as Botryococcus braunii.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.1 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100008en10.4067/S0717-34582007000100008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	biomass carbon dioxide carotenoids fatty acids inter simple sequence repeats saturated hydrocarbons
spellingShingle	biomass carbon dioxide carotenoids fatty acids inter simple sequence repeats saturated hydrocarbons Dayananda,Chandrappa Sarada,Ravi Kumar,Vinod Ravishankar,Gokare Aswathanarayana Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies
description	Samples of green colonial unicellular microalga Botryococcus braunii were collected from Bear Shola Falls at Kodaikanal (latitude 10.31 N and longitude 77.32 E), Tamil Nadu, India. Specimens were isolated, cultured and examined for its hydrocarbon content, morphological features and DNA structural resemblance with the known strain to confirm its identity. Inter simple sequence repeats (ISSR) finger printing revealed strong genetic similarity among the authentic strain (B. braunii N-836) and the Indian isolated strain (B. braunii CFTRI- Bb1) from Kodaikanal. The type of hydrocarbons produced by the Kodaikanal isolates were analyzed and identified as saturated hydrocarbons in the range of C21 to C33 by GCMS. Tetracosane and octacosane were found as the major components among the saturated hydrocarbons produced by this alga, constituting 17.6% and 14.8% respectively. Hydrocarbon content of the organism was in the range of 13-18% of its dry biomass. The fat content of the organism was found to be 22% (w/w). Palmitic and oleic acids were found to be major fatty acids produced by the alga. Lutein and β-carotene were found to be the major carotenoids and constituted about 64.1% and 25.1% respectively of the total carotenoids. Based on ISSR finger printing and hydrocarbon analyses the new algal strain from Kodaikanal was identified as Botryococcus braunii.
author	Dayananda,Chandrappa Sarada,Ravi Kumar,Vinod Ravishankar,Gokare Aswathanarayana
author_facet	Dayananda,Chandrappa Sarada,Ravi Kumar,Vinod Ravishankar,Gokare Aswathanarayana
author_sort	Dayananda,Chandrappa
title	Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies
title_short	Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies
title_full	Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies
title_fullStr	Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies
title_full_unstemmed	Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies
title_sort	isolation and characterization of hydrocarbon producing green alga botryococcus braunii from indian freshwater bodies
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100008
work_keys_str_mv	AT dayanandachandrappa isolationandcharacterizationofhydrocarbonproducinggreenalgabotryococcusbrauniifromindianfreshwaterbodies AT saradaravi isolationandcharacterizationofhydrocarbonproducinggreenalgabotryococcusbrauniifromindianfreshwaterbodies AT kumarvinod isolationandcharacterizationofhydrocarbonproducinggreenalgabotryococcusbrauniifromindianfreshwaterbodies AT ravishankargokareaswathanarayana isolationandcharacterizationofhydrocarbonproducinggreenalgabotryococcusbrauniifromindianfreshwaterbodies
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Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies

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