Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers

A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repe...

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Autores principales:	Lakshmanan,Venkatachalam, Reddampalli Venkataramareddy,Sreedhar, Neelwarne,Bhagyalakshmi
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	Growth regulators Musa protocol shoot cultures somaclonal variation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100010
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spelling	oai:scielo:S0717-345820070001000102007-08-14Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markersLakshmanan,VenkatachalamReddampalli Venkataramareddy,SreedharNeelwarne,Bhagyalakshmi Growth regulators Musa protocol shoot cultures somaclonal variation A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro plantlets, 11 were used for screening that involved shoot cultures with distinct variation in morphological characteristics (morphotypes). Similarly, the mother maintained in the field was also subjected for genetic analysis. Out of the 50 RAPD and 25 ISSR primers screened, 30 RAPD and 5 ISSR primers produced totally 424 clear, distinct and reproducible band classes resulting in a total of 5088 bands where the banding patterns for each primer was highly uniform and comparable to the field-grown mother clone from which the cultures had been established. These results indicate that the micropropagation protocol developed by us for rapid in vitro multiplication is appropriate and applicable for clonal propagation of banana var. NR over a long period. This is the first report on the use of genetic markers to establish genetic fidelity of long-term micropropagated banana using RAPD and ISSR.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.1 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100010en10.4067/S0717-34582007000100010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Growth regulators Musa protocol shoot cultures somaclonal variation
spellingShingle	Growth regulators Musa protocol shoot cultures somaclonal variation Lakshmanan,Venkatachalam Reddampalli Venkataramareddy,Sreedhar Neelwarne,Bhagyalakshmi Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers
description	A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro plantlets, 11 were used for screening that involved shoot cultures with distinct variation in morphological characteristics (morphotypes). Similarly, the mother maintained in the field was also subjected for genetic analysis. Out of the 50 RAPD and 25 ISSR primers screened, 30 RAPD and 5 ISSR primers produced totally 424 clear, distinct and reproducible band classes resulting in a total of 5088 bands where the banding patterns for each primer was highly uniform and comparable to the field-grown mother clone from which the cultures had been established. These results indicate that the micropropagation protocol developed by us for rapid in vitro multiplication is appropriate and applicable for clonal propagation of banana var. NR over a long period. This is the first report on the use of genetic markers to establish genetic fidelity of long-term micropropagated banana using RAPD and ISSR.
author	Lakshmanan,Venkatachalam Reddampalli Venkataramareddy,Sreedhar Neelwarne,Bhagyalakshmi
author_facet	Lakshmanan,Venkatachalam Reddampalli Venkataramareddy,Sreedhar Neelwarne,Bhagyalakshmi
author_sort	Lakshmanan,Venkatachalam
title	Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers
title_short	Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers
title_full	Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers
title_fullStr	Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers
title_full_unstemmed	Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers
title_sort	molecular analysis of genetic stability in long-term micropropagated shoots of banana using rapd and issr markers
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100010
work_keys_str_mv	AT lakshmananvenkatachalam molecularanalysisofgeneticstabilityinlongtermmicropropagatedshootsofbananausingrapdandissrmarkers AT reddampallivenkataramareddysreedhar molecularanalysisofgeneticstabilityinlongtermmicropropagatedshootsofbananausingrapdandissrmarkers AT neelwarnebhagyalakshmi molecularanalysisofgeneticstabilityinlongtermmicropropagatedshootsofbananausingrapdandissrmarkers
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Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers

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