Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant

Camptothecin (CPT) analogues and derivatives serve as a novel class of effective anticancer agents that exert their action against DNA topoisomerase I. This paper presents procedures for the rapid, high frequency regeneration of a camptothecin producing plant, Ophiorrhiza prostrata D. Don from leaf...

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Autores principales:	Shahanaz Beegum,A, Poulose Martin,Kottackal, Zhang,Chun-Lai, Nishitha,I. K., Ligimol, Slater,Adrian, Madhusoodanan,P. V
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	camptothecin medicinal plant Ophiorrhiza prostrata plant growth regulators
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100011
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spelling	oai:scielo:S0717-345820070001000112007-08-14Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plantShahanaz Beegum,APoulose Martin,KottackalZhang,Chun-LaiNishitha,I. K.Ligimol,Slater,AdrianMadhusoodanan,P. V camptothecin medicinal plant Ophiorrhiza prostrata plant growth regulators Camptothecin (CPT) analogues and derivatives serve as a novel class of effective anticancer agents that exert their action against DNA topoisomerase I. This paper presents procedures for the rapid, high frequency regeneration of a camptothecin producing plant, Ophiorrhiza prostrata D. Don from leaf and internode explants via shoot organogenesis. The concentrations of plant growth regulators and explant types exhibited discrete roles in the efficacy of shoot induction. N6-benzyladenine (BA) was the most effective cytokinin for the induction of shoots. Murashige and Skoog (MS) medium with 8.87 µM BA and 2.46 µM indole-3-butyric acid (IBA) yielded the highest number of shoots from leaf and internode explants (76.0 and 90.8 shoots respectively). In the case of leaf explants, explants from the proximal end produced a higher number of shoots than those from the mid and distal end. Leaf and internode explants cultured on MS medium supplemented with α-naphthaleneacetic acid (NAA) and BA developed shoots, calli and roots. Calli subcultured onto medium supplemented with 8.87 µM BA and 2.46 µM IBA developed a mean of 20.1 shoots within 40 days. Excision and culture of internode and proximal leaf explants from the established cultures on MS basal medium significantly enhanced the number of shoots and yielded a mean of 18.3 and 13.7 shoots respectively within 40 days. Histological examination of leaf explants showed that the shoots were of sub-epidermal origin, confined to the sub-epidermal cells above the vascular traces. Shoots cultured on half-strength MS basal medium with 10.74 µM NAA and 2.32 µM Kn produced a mean of 48.2 roots per shoot. Direct transfer of rootless healthy shoots showed a 50% survival rate, whilst it was 100 percent in the case of in vitro rooted shoots.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.1 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100011en10.4067/S0717-34582007000100011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	camptothecin medicinal plant Ophiorrhiza prostrata plant growth regulators
spellingShingle	camptothecin medicinal plant Ophiorrhiza prostrata plant growth regulators Shahanaz Beegum,A Poulose Martin,Kottackal Zhang,Chun-Lai Nishitha,I. K. Ligimol, Slater,Adrian Madhusoodanan,P. V Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant
description	Camptothecin (CPT) analogues and derivatives serve as a novel class of effective anticancer agents that exert their action against DNA topoisomerase I. This paper presents procedures for the rapid, high frequency regeneration of a camptothecin producing plant, Ophiorrhiza prostrata D. Don from leaf and internode explants via shoot organogenesis. The concentrations of plant growth regulators and explant types exhibited discrete roles in the efficacy of shoot induction. N6-benzyladenine (BA) was the most effective cytokinin for the induction of shoots. Murashige and Skoog (MS) medium with 8.87 µM BA and 2.46 µM indole-3-butyric acid (IBA) yielded the highest number of shoots from leaf and internode explants (76.0 and 90.8 shoots respectively). In the case of leaf explants, explants from the proximal end produced a higher number of shoots than those from the mid and distal end. Leaf and internode explants cultured on MS medium supplemented with α-naphthaleneacetic acid (NAA) and BA developed shoots, calli and roots. Calli subcultured onto medium supplemented with 8.87 µM BA and 2.46 µM IBA developed a mean of 20.1 shoots within 40 days. Excision and culture of internode and proximal leaf explants from the established cultures on MS basal medium significantly enhanced the number of shoots and yielded a mean of 18.3 and 13.7 shoots respectively within 40 days. Histological examination of leaf explants showed that the shoots were of sub-epidermal origin, confined to the sub-epidermal cells above the vascular traces. Shoots cultured on half-strength MS basal medium with 10.74 µM NAA and 2.32 µM Kn produced a mean of 48.2 roots per shoot. Direct transfer of rootless healthy shoots showed a 50% survival rate, whilst it was 100 percent in the case of in vitro rooted shoots.
author	Shahanaz Beegum,A Poulose Martin,Kottackal Zhang,Chun-Lai Nishitha,I. K. Ligimol, Slater,Adrian Madhusoodanan,P. V
author_facet	Shahanaz Beegum,A Poulose Martin,Kottackal Zhang,Chun-Lai Nishitha,I. K. Ligimol, Slater,Adrian Madhusoodanan,P. V
author_sort	Shahanaz Beegum,A
title	Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant
title_short	Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant
title_full	Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant
title_fullStr	Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant
title_full_unstemmed	Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant
title_sort	organogenesis from leaf and internode explants of ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100011
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Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant

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