Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solution
The lysozyme enzyme was immobilized on vitreous surface (fragments with diameters of 0.3 and 1.0 mm) for remediation of the microorganism Escherichia coli JM 109 into fresh water and saline solutions with 0.9% NaCl (w/v). Characterization of enzymatic film was carried out by infrared spectroscopy an...
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Pontificia Universidad Católica de Valparaíso
2007
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oai:scielo:S0717-345820070001000152007-08-14Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solutionAlves,Carlucio RPimenta,Maria Gardenny RVieira,Regine H.S.FFurtado,Roselayne FGuedes,Maria Izabel FSilva,Rui C.BAssis,Odilio B.G Escherichia coli lysozyme self-assembly The lysozyme enzyme was immobilized on vitreous surface (fragments with diameters of 0.3 and 1.0 mm) for remediation of the microorganism Escherichia coli JM 109 into fresh water and saline solutions with 0.9% NaCl (w/v). Characterization of enzymatic film was carried out by infrared spectroscopy and atomic force microscopy techniques. Bactericide activity of the enzyme was evaluated by spectrophotometric analysis. It was verified that the enzymatic film was strongly coupled with the vitreous surface. The topographic analysis demonstrated that the deposited film was uniform and homogeneous. It was observed bactericide activity of film deposited on vitreous surface with 0.3 mm in fresh and saline solutions. This fact was not verified to vitreous fragments with 1.0 mm of diameterinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.1 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100015en10.4067/S0717-34582007000100015 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Escherichia coli lysozyme self-assembly |
| spellingShingle |
Escherichia coli lysozyme self-assembly Alves,Carlucio R Pimenta,Maria Gardenny R Vieira,Regine H.S.F Furtado,Roselayne F Guedes,Maria Izabel F Silva,Rui C.B Assis,Odilio B.G Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solution |
| description |
The lysozyme enzyme was immobilized on vitreous surface (fragments with diameters of 0.3 and 1.0 mm) for remediation of the microorganism Escherichia coli JM 109 into fresh water and saline solutions with 0.9% NaCl (w/v). Characterization of enzymatic film was carried out by infrared spectroscopy and atomic force microscopy techniques. Bactericide activity of the enzyme was evaluated by spectrophotometric analysis. It was verified that the enzymatic film was strongly coupled with the vitreous surface. The topographic analysis demonstrated that the deposited film was uniform and homogeneous. It was observed bactericide activity of film deposited on vitreous surface with 0.3 mm in fresh and saline solutions. This fact was not verified to vitreous fragments with 1.0 mm of diameter |
| author |
Alves,Carlucio R Pimenta,Maria Gardenny R Vieira,Regine H.S.F Furtado,Roselayne F Guedes,Maria Izabel F Silva,Rui C.B Assis,Odilio B.G |
| author_facet |
Alves,Carlucio R Pimenta,Maria Gardenny R Vieira,Regine H.S.F Furtado,Roselayne F Guedes,Maria Izabel F Silva,Rui C.B Assis,Odilio B.G |
| author_sort |
Alves,Carlucio R |
| title |
Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solution |
| title_short |
Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solution |
| title_full |
Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solution |
| title_fullStr |
Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solution |
| title_full_unstemmed |
Practical use of immobilized lysozyme for the remediation process of Escherichia coli in aqueous solution |
| title_sort |
practical use of immobilized lysozyme for the remediation process of escherichia coli in aqueous solution |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2007 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100015 |
| work_keys_str_mv |
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| _version_ |
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