A practical approach to the understanding and teaching of RNA silencing in plants

Gene silencing, also called RNA interference (RNAi) is a specific mechanism of RNA degradation involved in gene regulation, development and defense in eukaryotic organisms. It became an important subject in the teaching programs of molecular biology, genetics and biotechnology courses in the last ye...

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Autores principales:	Bazzini,Ariel A, Mongelli,Vanesa C, Hopp,H. Esteban, del Vas,Mariana, Asurmendi,Sebastián
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	gene silencing GFP HC-Pro PDS protocols PTGS teaching transgenic transient expression TRV VIGS
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200002
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spelling	oai:scielo:S0717-345820070002000022007-10-29A practical approach to the understanding and teaching of RNA silencing in plantsBazzini,Ariel AMongelli,Vanesa CHopp,H. Estebandel Vas,MarianaAsurmendi,Sebastián gene silencing GFP HC-Pro PDS protocols PTGS teaching transgenic transient expression TRV VIGS Gene silencing, also called RNA interference (RNAi) is a specific mechanism of RNA degradation involved in gene regulation, development and defense in eukaryotic organisms. It became an important subject in the teaching programs of molecular biology, genetics and biotechnology courses in the last years. The aim of this work is to provide simple and inexpensive assays to understand and teach gene silencing using plants as model systems. The use of transient and permanent transgenic plants for expressing reporter genes, like those derived from jellyfish green fluorescent protein (gfp) encoding gene, provides a nice, colorful and conclusive image of gene silencing. Three experimental approaches to evidence RNA silencing are depicted. In the first approach gene silencing is demonstrated after transient expression of reporter genes in non-transgenic plants. In the second, silencing is triggered against a reporter gene stably integrated into a transgenic plant. The third approach involves the triggering of RNA silencing against endogenous genes using viral vectors. In addition we illustrate systemic gene silencing showing how the silencing signal is spread over a plant and finally it is also demonstrated the suppression of gene silencing. The first group of experiments is recommended to be tough on undergraduate courses, the following two sections are recommended for graduate courses. Hopefully, it will help students to understand this important phenomenon and to unravel the importance of gene silencing as a key gene regulation mechanism and as a molecular and biotechnological tool.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.2 20072007-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200002en10.4067/S0717-34582007000200002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	gene silencing GFP HC-Pro PDS protocols PTGS teaching transgenic transient expression TRV VIGS
spellingShingle	gene silencing GFP HC-Pro PDS protocols PTGS teaching transgenic transient expression TRV VIGS Bazzini,Ariel A Mongelli,Vanesa C Hopp,H. Esteban del Vas,Mariana Asurmendi,Sebastián A practical approach to the understanding and teaching of RNA silencing in plants
description	Gene silencing, also called RNA interference (RNAi) is a specific mechanism of RNA degradation involved in gene regulation, development and defense in eukaryotic organisms. It became an important subject in the teaching programs of molecular biology, genetics and biotechnology courses in the last years. The aim of this work is to provide simple and inexpensive assays to understand and teach gene silencing using plants as model systems. The use of transient and permanent transgenic plants for expressing reporter genes, like those derived from jellyfish green fluorescent protein (gfp) encoding gene, provides a nice, colorful and conclusive image of gene silencing. Three experimental approaches to evidence RNA silencing are depicted. In the first approach gene silencing is demonstrated after transient expression of reporter genes in non-transgenic plants. In the second, silencing is triggered against a reporter gene stably integrated into a transgenic plant. The third approach involves the triggering of RNA silencing against endogenous genes using viral vectors. In addition we illustrate systemic gene silencing showing how the silencing signal is spread over a plant and finally it is also demonstrated the suppression of gene silencing. The first group of experiments is recommended to be tough on undergraduate courses, the following two sections are recommended for graduate courses. Hopefully, it will help students to understand this important phenomenon and to unravel the importance of gene silencing as a key gene regulation mechanism and as a molecular and biotechnological tool.
author	Bazzini,Ariel A Mongelli,Vanesa C Hopp,H. Esteban del Vas,Mariana Asurmendi,Sebastián
author_facet	Bazzini,Ariel A Mongelli,Vanesa C Hopp,H. Esteban del Vas,Mariana Asurmendi,Sebastián
author_sort	Bazzini,Ariel A
title	A practical approach to the understanding and teaching of RNA silencing in plants
title_short	A practical approach to the understanding and teaching of RNA silencing in plants
title_full	A practical approach to the understanding and teaching of RNA silencing in plants
title_fullStr	A practical approach to the understanding and teaching of RNA silencing in plants
title_full_unstemmed	A practical approach to the understanding and teaching of RNA silencing in plants
title_sort	practical approach to the understanding and teaching of rna silencing in plants
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200002
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A practical approach to the understanding and teaching of RNA silencing in plants

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