Insect resistance and risk assessment studies of advanced generations of basmati rice expressing two genes of Bacillus thuringiensis

Advanced generations of different transgenic lines of indica basmati rice (Basmati-370) expressing two unrelated Bt genes, cry1Ac and cry2A were evaluated for resistance to Yellow Stem Borer (YSB) and Rice Leaf Folder (RLF) under field conditions compared to control lines over three years (2003-2005...

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Autores principales: Rahman,Mahmood-ur, Rashid,Hamza, Shahid,Ahmad Ali, Bashir,Khurram, Husnain,Tayyab, Riazuddin,Sheikh
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2007
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200008
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spelling oai:scielo:S0717-345820070002000082007-10-29Insect resistance and risk assessment studies of advanced generations of basmati rice expressing two genes of Bacillus thuringiensisRahman,Mahmood-urRashid,HamzaShahid,Ahmad AliBashir,KhurramHusnain,TayyabRiazuddin,Sheikh bio-safety cry1Ac cry2A gene flow field trials gene pyramiding insect resistance Advanced generations of different transgenic lines of indica basmati rice (Basmati-370) expressing two unrelated Bt genes, cry1Ac and cry2A were evaluated for resistance to Yellow Stem Borer (YSB) and Rice Leaf Folder (RLF) under field conditions compared to control lines over three years (2003-2005). Homozygous lines were selected and analyzed for insect resistance, morphological, physiochemical properties and risk assessment studies. After artificial infestation of target insects, the transgenic plants showed significant resistance. Data were recorded in terms of dead hearts and white heads at vegetative and flowering stage respectively. Transgenic lines showed up to 100 and 96% resistance against yellow stem borer at vegetative and flowering stages, respectively. Natural damage of rice leaf folder was also observed during the year 2005. The transgenic plants were 98% more resistant as compared to untransformed control plants. Variations in some morphological characteristics, e.g., the average number of tillers, plant height and maturity were also observed. Transgenic lines produced 40% more grains than control plants. All these characteristics were stably inherited in advanced generations. The transgenic lines had no significant effect on non-target insects (insects belonging to orders other than Lepidoptera and Diptera) in field or under storage conditions. Chances of pollen-mediated gene flow were recorded at a rate of 0.14%info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.2 20072007-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200008en10.4067/S0717-34582007000200008
institution Scielo Chile
collection Scielo Chile
language English
topic bio-safety
cry1Ac
cry2A
gene flow
field trials
gene pyramiding
insect resistance
spellingShingle bio-safety
cry1Ac
cry2A
gene flow
field trials
gene pyramiding
insect resistance
Rahman,Mahmood-ur
Rashid,Hamza
Shahid,Ahmad Ali
Bashir,Khurram
Husnain,Tayyab
Riazuddin,Sheikh
Insect resistance and risk assessment studies of advanced generations of basmati rice expressing two genes of Bacillus thuringiensis
description Advanced generations of different transgenic lines of indica basmati rice (Basmati-370) expressing two unrelated Bt genes, cry1Ac and cry2A were evaluated for resistance to Yellow Stem Borer (YSB) and Rice Leaf Folder (RLF) under field conditions compared to control lines over three years (2003-2005). Homozygous lines were selected and analyzed for insect resistance, morphological, physiochemical properties and risk assessment studies. After artificial infestation of target insects, the transgenic plants showed significant resistance. Data were recorded in terms of dead hearts and white heads at vegetative and flowering stage respectively. Transgenic lines showed up to 100 and 96% resistance against yellow stem borer at vegetative and flowering stages, respectively. Natural damage of rice leaf folder was also observed during the year 2005. The transgenic plants were 98% more resistant as compared to untransformed control plants. Variations in some morphological characteristics, e.g., the average number of tillers, plant height and maturity were also observed. Transgenic lines produced 40% more grains than control plants. All these characteristics were stably inherited in advanced generations. The transgenic lines had no significant effect on non-target insects (insects belonging to orders other than Lepidoptera and Diptera) in field or under storage conditions. Chances of pollen-mediated gene flow were recorded at a rate of 0.14%
author Rahman,Mahmood-ur
Rashid,Hamza
Shahid,Ahmad Ali
Bashir,Khurram
Husnain,Tayyab
Riazuddin,Sheikh
author_facet Rahman,Mahmood-ur
Rashid,Hamza
Shahid,Ahmad Ali
Bashir,Khurram
Husnain,Tayyab
Riazuddin,Sheikh
author_sort Rahman,Mahmood-ur
title Insect resistance and risk assessment studies of advanced generations of basmati rice expressing two genes of Bacillus thuringiensis
title_short Insect resistance and risk assessment studies of advanced generations of basmati rice expressing two genes of Bacillus thuringiensis
title_full Insect resistance and risk assessment studies of advanced generations of basmati rice expressing two genes of Bacillus thuringiensis
title_fullStr Insect resistance and risk assessment studies of advanced generations of basmati rice expressing two genes of Bacillus thuringiensis
title_full_unstemmed Insect resistance and risk assessment studies of advanced generations of basmati rice expressing two genes of Bacillus thuringiensis
title_sort insect resistance and risk assessment studies of advanced generations of basmati rice expressing two genes of bacillus thuringiensis
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2007
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200008
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AT shahidahmadali insectresistanceandriskassessmentstudiesofadvancedgenerationsofbasmatiriceexpressingtwogenesofbacillusthuringiensis
AT bashirkhurram insectresistanceandriskassessmentstudiesofadvancedgenerationsofbasmatiriceexpressingtwogenesofbacillusthuringiensis
AT husnaintayyab insectresistanceandriskassessmentstudiesofadvancedgenerationsofbasmatiriceexpressingtwogenesofbacillusthuringiensis
AT riazuddinsheikh insectresistanceandriskassessmentstudiesofadvancedgenerationsofbasmatiriceexpressingtwogenesofbacillusthuringiensis
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