Membrane damage of bacteria by silanols treatment

Antimicrobial action of silanols, a new class of antimicrobials, was investigated by transmission electron microscopy and fluorescent dye studies. Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa and Gram-positive bacteria, Staphylococcus aureus and Enterococcus faecalis were trea...

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Autores principales:	Kim,Yun-mi, Farrah,Samuel, Baney,Ronald H
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	antimicrobials hydrophobicity organosilicon compounds
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200009
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spelling	oai:scielo:S0717-345820070002000092007-10-29Membrane damage of bacteria by silanols treatmentKim,Yun-miFarrah,SamuelBaney,Ronald H antimicrobials hydrophobicity organosilicon compounds Antimicrobial action of silanols, a new class of antimicrobials, was investigated by transmission electron microscopy and fluorescent dye studies. Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa and Gram-positive bacteria, Staphylococcus aureus and Enterococcus faecalis were treated by silanols at concentration of less than 0.2 wt% for one hour. Membrane damage of the bacteria by the silanol treatment was clearly observed by transmission electron microscopy. Separation of the cytoplasmic membrane from the outer membrane for E. coli and disorganized cytoplasmic membrane of the Gram-positive bacteria were observed when compared to the control. Fluorescent dyes, green-fluorescent nucleic acid stain (Syto 9) and the red-fluorescent nucleic acid stain (Propidium iodide), were used to monitor membrane damage of the bacteria by Confocal microscopy and Spectrophotometer. A reduction of the green fluorescent emission was detected for silanol treated bacteria indicating membrane damage of the bacteria and supporting the hypothesis that their viability loss may be due to their membrane damage analogus to alcoholsinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.2 20072007-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200009en10.4067/S0717-34582007000200009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	antimicrobials hydrophobicity organosilicon compounds
spellingShingle	antimicrobials hydrophobicity organosilicon compounds Kim,Yun-mi Farrah,Samuel Baney,Ronald H Membrane damage of bacteria by silanols treatment
description	Antimicrobial action of silanols, a new class of antimicrobials, was investigated by transmission electron microscopy and fluorescent dye studies. Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa and Gram-positive bacteria, Staphylococcus aureus and Enterococcus faecalis were treated by silanols at concentration of less than 0.2 wt% for one hour. Membrane damage of the bacteria by the silanol treatment was clearly observed by transmission electron microscopy. Separation of the cytoplasmic membrane from the outer membrane for E. coli and disorganized cytoplasmic membrane of the Gram-positive bacteria were observed when compared to the control. Fluorescent dyes, green-fluorescent nucleic acid stain (Syto 9) and the red-fluorescent nucleic acid stain (Propidium iodide), were used to monitor membrane damage of the bacteria by Confocal microscopy and Spectrophotometer. A reduction of the green fluorescent emission was detected for silanol treated bacteria indicating membrane damage of the bacteria and supporting the hypothesis that their viability loss may be due to their membrane damage analogus to alcohols
author	Kim,Yun-mi Farrah,Samuel Baney,Ronald H
author_facet	Kim,Yun-mi Farrah,Samuel Baney,Ronald H
author_sort	Kim,Yun-mi
title	Membrane damage of bacteria by silanols treatment
title_short	Membrane damage of bacteria by silanols treatment
title_full	Membrane damage of bacteria by silanols treatment
title_fullStr	Membrane damage of bacteria by silanols treatment
title_full_unstemmed	Membrane damage of bacteria by silanols treatment
title_sort	membrane damage of bacteria by silanols treatment
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200009
work_keys_str_mv	AT kimyunmi membranedamageofbacteriabysilanolstreatment AT farrahsamuel membranedamageofbacteriabysilanolstreatment AT baneyronaldh membranedamageofbacteriabysilanolstreatment
_version_	1718441767274020864

Membrane damage of bacteria by silanols treatment

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