Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922

This study reports the purification and characterization of ß-glucosidase from a newly isolated thermophilic fungus, Melanocarpus sp. Microbial Type Culture Collection (MTCC) 3922. The molecular weight of ß-glucosidase was determined to be ~ 92 and 102 kDa with SDS PAGE and gel filtration, respectiv...

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Autores principales:	Kaur,Jatinder, Chadha,Bhupinder S, Kumar,Badhan A, Kaur,Ghatora, S, Saini,Harvinder S
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	ß-glucosidase Melanocarpus sp purification substrate specificity transglycosylation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200010
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spelling	oai:scielo:S0717-345820070002000102007-10-29Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922Kaur,JatinderChadha,Bhupinder SKumar,Badhan AKaur,Ghatora, SSaini,Harvinder S ß-glucosidase Melanocarpus sp purification substrate specificity transglycosylation This study reports the purification and characterization of ß-glucosidase from a newly isolated thermophilic fungus, Melanocarpus sp. Microbial Type Culture Collection (MTCC) 3922. The molecular weight of ß-glucosidase was determined to be ~ 92 and 102 kDa with SDS PAGE and gel filtration, respectively, and pI of ~ 4.1. It was optimally active at 60ºC and pH 6.0, though was stable at 50ºC and pH 5.0 - 6.0. The presence of DTT, mercaptoethanol and metal ions such as Na+, K+, Ca2+, Mg2+and Zn2+ positively influenced the activity of ß-glucosidase but the activity was inhibited in the presence of CuSO4. ß-Glucosidase recognized pNP- ß-glucopyranoside (pNPG) as the preferred substrate, and showed very low affinity for pNP- ß-D-cellobioside. Km and Vmax for the hydrolysis of pNPG by ß-glucosidase was calculated as 3.3 mM and 43.68 µmolmin-1mg protein-1, respectively and k cat was quantified as 4 x 10³ min-1. ß-Glucosidase activity was enhanced appreciably in the presence of alcohols (methanol and ethanol) moreover, purified ß-glucosidase showed putative transglycosylation activity that was positively catalyzed in presence of methanol as an acceptor moleculeinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.2 20072007-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200010en10.4067/S0717-34582007000200010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	ß-glucosidase Melanocarpus sp purification substrate specificity transglycosylation
spellingShingle	ß-glucosidase Melanocarpus sp purification substrate specificity transglycosylation Kaur,Jatinder Chadha,Bhupinder S Kumar,Badhan A Kaur,Ghatora, S Saini,Harvinder S Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922
description	This study reports the purification and characterization of ß-glucosidase from a newly isolated thermophilic fungus, Melanocarpus sp. Microbial Type Culture Collection (MTCC) 3922. The molecular weight of ß-glucosidase was determined to be ~ 92 and 102 kDa with SDS PAGE and gel filtration, respectively, and pI of ~ 4.1. It was optimally active at 60ºC and pH 6.0, though was stable at 50ºC and pH 5.0 - 6.0. The presence of DTT, mercaptoethanol and metal ions such as Na+, K+, Ca2+, Mg2+and Zn2+ positively influenced the activity of ß-glucosidase but the activity was inhibited in the presence of CuSO4. ß-Glucosidase recognized pNP- ß-glucopyranoside (pNPG) as the preferred substrate, and showed very low affinity for pNP- ß-D-cellobioside. Km and Vmax for the hydrolysis of pNPG by ß-glucosidase was calculated as 3.3 mM and 43.68 µmolmin-1mg protein-1, respectively and k cat was quantified as 4 x 10³ min-1. ß-Glucosidase activity was enhanced appreciably in the presence of alcohols (methanol and ethanol) moreover, purified ß-glucosidase showed putative transglycosylation activity that was positively catalyzed in presence of methanol as an acceptor molecule
author	Kaur,Jatinder Chadha,Bhupinder S Kumar,Badhan A Kaur,Ghatora, S Saini,Harvinder S
author_facet	Kaur,Jatinder Chadha,Bhupinder S Kumar,Badhan A Kaur,Ghatora, S Saini,Harvinder S
author_sort	Kaur,Jatinder
title	Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922
title_short	Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922
title_full	Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922
title_fullStr	Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922
title_full_unstemmed	Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922
title_sort	purification and characterization of ß-glucosidase from melanocarpus sp. mtcc 3922
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200010
work_keys_str_mv	AT kaurjatinder purificationandcharacterizationofßglucosidasefrommelanocarpusspmtcc3922 AT chadhabhupinders purificationandcharacterizationofßglucosidasefrommelanocarpusspmtcc3922 AT kumarbadhana purificationandcharacterizationofßglucosidasefrommelanocarpusspmtcc3922 AT kaurghatoras purificationandcharacterizationofßglucosidasefrommelanocarpusspmtcc3922 AT sainiharvinders purificationandcharacterizationofßglucosidasefrommelanocarpusspmtcc3922
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Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922

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