Peptide synthesis: chemical or enzymatic

Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the adva...

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Autores principales:	Guzmán,Fanny, Barberis,Sonia, Illanes,Andrés
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	enzymatic synthesis peptides proteases solid-phase synthesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200012
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spelling	oai:scielo:S0717-345820070002000122007-10-29Peptide synthesis: chemical or enzymaticGuzmán,FannyBarberis,SoniaIllanes,Andrés enzymatic synthesis peptides proteases solid-phase synthesis Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis is the most mature technology for peptide synthesis, lack of specificity and environmental burden are severe drawbacks that can in principle be successfully overcame by enzyme biocatalysis. However, productivity of enzymatic synthesis is lower, costs of biocatalysts are usually high and no protocols exist for its validation and scale-up, representing challenges that are being actively confronted by intense research and development in this area. The combination of chemical and enzymatic synthesis is probably the way to go, since the good properties of each technology can be synergistically used in the context of one process objective.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.2 20072007-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200012en10.4067/S0717-34582007000200012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	enzymatic synthesis peptides proteases solid-phase synthesis
spellingShingle	enzymatic synthesis peptides proteases solid-phase synthesis Guzmán,Fanny Barberis,Sonia Illanes,Andrés Peptide synthesis: chemical or enzymatic
description	Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis is the most mature technology for peptide synthesis, lack of specificity and environmental burden are severe drawbacks that can in principle be successfully overcame by enzyme biocatalysis. However, productivity of enzymatic synthesis is lower, costs of biocatalysts are usually high and no protocols exist for its validation and scale-up, representing challenges that are being actively confronted by intense research and development in this area. The combination of chemical and enzymatic synthesis is probably the way to go, since the good properties of each technology can be synergistically used in the context of one process objective.
author	Guzmán,Fanny Barberis,Sonia Illanes,Andrés
author_facet	Guzmán,Fanny Barberis,Sonia Illanes,Andrés
author_sort	Guzmán,Fanny
title	Peptide synthesis: chemical or enzymatic
title_short	Peptide synthesis: chemical or enzymatic
title_full	Peptide synthesis: chemical or enzymatic
title_fullStr	Peptide synthesis: chemical or enzymatic
title_full_unstemmed	Peptide synthesis: chemical or enzymatic
title_sort	peptide synthesis: chemical or enzymatic
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200012
work_keys_str_mv	AT guzmanfanny peptidesynthesischemicalorenzymatic AT barberissonia peptidesynthesischemicalorenzymatic AT illanesandres peptidesynthesischemicalorenzymatic
_version_	1718441767931478016

Peptide synthesis: chemical or enzymatic

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