Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter

A novel approach for functional complementation of foreign genes in Saccharomyces cerevisiae is presented. This approach is based on the use of the widely available cognate gene plasmids (e.g. pRS416) of the European Functional Analysis Network (EUROFAN). The functional complementation of the human...

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Autores principales:	Lavigne,Rob, Volckaert,Guido
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	cognate gene plasmid, functional complementation homolog Saccharomyces cerevisiae yeast
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200014
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spelling	oai:scielo:S0717-345820070002000142007-10-29Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoterLavigne,RobVolckaert,Guido cognate gene plasmid, functional complementation homolog Saccharomyces cerevisiae yeast A novel approach for functional complementation of foreign genes in Saccharomyces cerevisiae is presented. This approach is based on the use of the widely available cognate gene plasmids (e.g. pRS416) of the European Functional Analysis Network (EUROFAN). The functional complementation of the human homolog of YOR159c (SME1 gene) shown here is the first demonstration of complementation using the original yeast promoter, theoretically offering a more natural regulation of protein expressioninfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.2 20072007-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200014en10.4067/S0717-34582007000200014
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cognate gene plasmid, functional complementation homolog Saccharomyces cerevisiae yeast
spellingShingle	cognate gene plasmid, functional complementation homolog Saccharomyces cerevisiae yeast Lavigne,Rob Volckaert,Guido Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter
description	A novel approach for functional complementation of foreign genes in Saccharomyces cerevisiae is presented. This approach is based on the use of the widely available cognate gene plasmids (e.g. pRS416) of the European Functional Analysis Network (EUROFAN). The functional complementation of the human homolog of YOR159c (SME1 gene) shown here is the first demonstration of complementation using the original yeast promoter, theoretically offering a more natural regulation of protein expression
author	Lavigne,Rob Volckaert,Guido
author_facet	Lavigne,Rob Volckaert,Guido
author_sort	Lavigne,Rob
title	Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter
title_short	Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter
title_full	Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter
title_fullStr	Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter
title_full_unstemmed	Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter
title_sort	functional complementation in saccharomyces cerevisiae under control of the natural yeast promoter
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200014
work_keys_str_mv	AT lavignerob functionalcomplementationinsaccharomycescerevisiaeundercontrolofthenaturalyeastpromoter AT volckaertguido functionalcomplementationinsaccharomycescerevisiaeundercontrolofthenaturalyeastpromoter
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Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter

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