Single embryoid body formation in a multi-well plate

Embryoid bodies (EB) formed from murine embryonic stem (ES) cells recapitulate many aspects of a developing embryo. Of specific importance, synchronous differentiation of EB recapitulates organ-specific development and is achieved in culture by formation of uniformly sized EB. The method described h...

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Autores principales:	Ezekiel,Uthayashanker R, Muthuchamy,Mariappan, Ryerse,Jan S, Heuertz,Rita M
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	differentiation EB ES murine embryonic stem cell
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200015
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spelling	oai:scielo:S0717-345820070002000152007-10-29Single embryoid body formation in a multi-well plateEzekiel,Uthayashanker RMuthuchamy,MariappanRyerse,Jan SHeuertz,Rita M differentiation EB ES murine embryonic stem cell Embryoid bodies (EB) formed from murine embryonic stem (ES) cells recapitulate many aspects of a developing embryo. Of specific importance, synchronous differentiation of EB recapitulates organ-specific development and is achieved in culture by formation of uniformly sized EB. The method described here demonstrates a simple and cost-effective way of generating EB from murine ES cells. Single EB are formed in a multi-well plate format and large numbers of EB are generated using a 96-well multi-well plate. Uniform single-sized EB formed in the multi-well are an ideal system for screening compounds and determining differentiation effects. Since EB contain all three germ layers, they are appropriate for studying small molecule effects on differentiation of ES such as is performed in high-throughput screening protocolsinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.2 20072007-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200015en10.4067/S0717-34582007000200015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	differentiation EB ES murine embryonic stem cell
spellingShingle	differentiation EB ES murine embryonic stem cell Ezekiel,Uthayashanker R Muthuchamy,Mariappan Ryerse,Jan S Heuertz,Rita M Single embryoid body formation in a multi-well plate
description	Embryoid bodies (EB) formed from murine embryonic stem (ES) cells recapitulate many aspects of a developing embryo. Of specific importance, synchronous differentiation of EB recapitulates organ-specific development and is achieved in culture by formation of uniformly sized EB. The method described here demonstrates a simple and cost-effective way of generating EB from murine ES cells. Single EB are formed in a multi-well plate format and large numbers of EB are generated using a 96-well multi-well plate. Uniform single-sized EB formed in the multi-well are an ideal system for screening compounds and determining differentiation effects. Since EB contain all three germ layers, they are appropriate for studying small molecule effects on differentiation of ES such as is performed in high-throughput screening protocols
author	Ezekiel,Uthayashanker R Muthuchamy,Mariappan Ryerse,Jan S Heuertz,Rita M
author_facet	Ezekiel,Uthayashanker R Muthuchamy,Mariappan Ryerse,Jan S Heuertz,Rita M
author_sort	Ezekiel,Uthayashanker R
title	Single embryoid body formation in a multi-well plate
title_short	Single embryoid body formation in a multi-well plate
title_full	Single embryoid body formation in a multi-well plate
title_fullStr	Single embryoid body formation in a multi-well plate
title_full_unstemmed	Single embryoid body formation in a multi-well plate
title_sort	single embryoid body formation in a multi-well plate
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200015
work_keys_str_mv	AT ezekieluthayashankerr singleembryoidbodyformationinamultiwellplate AT muthuchamymariappan singleembryoidbodyformationinamultiwellplate AT ryersejans singleembryoidbodyformationinamultiwellplate AT heuertzritam singleembryoidbodyformationinamultiwellplate
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Single embryoid body formation in a multi-well plate

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