A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms

A molecular approach was used for selecting polyhydroxyalcanoate (PHA)-accumulating potential Gram-negative bacteria from different genera by colony polymerase chain reaction (PCR). Three degenerate primers were designed for amplifying a fragment from PHA synthase gene (phaC) (Class I), phaC1 and ph...

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Autores principales:	Revelo Romo,Dolly Margot, Grosso,María Victoria, Moreno Solano,Nubia Carmenza, Montoya Castaño,Dolly
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	16S rDNA colony PCR degenerate primers PHA synthase polyhydroxyalcanoate seminested PCR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300002
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spelling	oai:scielo:S0717-345820070003000022007-11-05A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganismsRevelo Romo,Dolly MargotGrosso,María VictoriaMoreno Solano,Nubia CarmenzaMontoya Castaño,Dolly 16S rDNA colony PCR degenerate primers PHA synthase polyhydroxyalcanoate seminested PCR A molecular approach was used for selecting polyhydroxyalcanoate (PHA)-accumulating potential Gram-negative bacteria from different genera by colony polymerase chain reaction (PCR). Three degenerate primers were designed for amplifying a fragment from PHA synthase gene (phaC) (Class I), phaC1 and phaC2 (Class II) genes for detecting PHA-producing bacteria. Thirty-four out of 55 bacterial strains from the old collection selected using Sudan black B staining were phaC+. PCR was used for directly selecting 35 new collection bacterial strains; these strains were phaC+ and their ability to produce PHA was confirmed by Sudan black B staining. Four specific primers were designed on genes of Class II PHA biosynthesis operon. These primers were used for evaluating 9 strains from the old phaC+ collection; 6 showed Class II PHA synthase organisation. 34 from the old and new bacterial isolation were characterised by 16S ribosomal gene (16S rDNA) gene partial sequencing. The tool proposed here can be used for better directing PHA production based on PHA biosynthesis genes and bacterial genera. Class I or II phaC genes were detected in 9 different genera and were able to infer the type of polymer produced.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.3 20072007-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300002en10.4067/S0717-34582007000300002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	16S rDNA colony PCR degenerate primers PHA synthase polyhydroxyalcanoate seminested PCR
spellingShingle	16S rDNA colony PCR degenerate primers PHA synthase polyhydroxyalcanoate seminested PCR Revelo Romo,Dolly Margot Grosso,María Victoria Moreno Solano,Nubia Carmenza Montoya Castaño,Dolly A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms
description	A molecular approach was used for selecting polyhydroxyalcanoate (PHA)-accumulating potential Gram-negative bacteria from different genera by colony polymerase chain reaction (PCR). Three degenerate primers were designed for amplifying a fragment from PHA synthase gene (phaC) (Class I), phaC1 and phaC2 (Class II) genes for detecting PHA-producing bacteria. Thirty-four out of 55 bacterial strains from the old collection selected using Sudan black B staining were phaC+. PCR was used for directly selecting 35 new collection bacterial strains; these strains were phaC+ and their ability to produce PHA was confirmed by Sudan black B staining. Four specific primers were designed on genes of Class II PHA biosynthesis operon. These primers were used for evaluating 9 strains from the old phaC+ collection; 6 showed Class II PHA synthase organisation. 34 from the old and new bacterial isolation were characterised by 16S ribosomal gene (16S rDNA) gene partial sequencing. The tool proposed here can be used for better directing PHA production based on PHA biosynthesis genes and bacterial genera. Class I or II phaC genes were detected in 9 different genera and were able to infer the type of polymer produced.
author	Revelo Romo,Dolly Margot Grosso,María Victoria Moreno Solano,Nubia Carmenza Montoya Castaño,Dolly
author_facet	Revelo Romo,Dolly Margot Grosso,María Victoria Moreno Solano,Nubia Carmenza Montoya Castaño,Dolly
author_sort	Revelo Romo,Dolly Margot
title	A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms
title_short	A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms
title_full	A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms
title_fullStr	A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms
title_full_unstemmed	A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms
title_sort	most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300002
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A most effective method for selecting a broad range of short and medium-chain-length polyhidroxyalcanoate producing microorganisms

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